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Literature summary extracted from

  • Ascano, A.; Nicholas, D.J.D.
    Purification and properties of O-acetyl-L-serine sulphhydrolase from wheat leaves (1977), Phytochemistry, 16, 889-893.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 5,5'-dithiobis(2-nitrobenzoic acid) non-competitive Triticum aestivum
2.5.1.47 L-cysteine non-competitive Triticum aestivum
2.5.1.47 L-homoserine non-competitive Triticum aestivum
2.5.1.47 methionine competitive to sulfide Triticum aestivum
2.5.1.47 NEM non-competitive Triticum aestivum
2.5.1.47 O-acetylserine above 72 mM Triticum aestivum
2.5.1.47 p-chloromercuribenzoate non-competitive Triticum aestivum
2.5.1.47 Sulfide above 107 mM Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.037
-
 O-acetyl-L-serine pH 6.8, 37°C Triticum aestivum
2.5.1.47 0.59
-
 H2S pH 6.8, 37°C Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 leaf
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 5.7
-
-
 Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
2.5.1.47 -15 C, phosphate buffer, pH 7.2, several months, no loss of activity Triticum aestivum
2.5.1.47 2 C, inactivated above, after a few days Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-serine
-
 Triticum aestivum L-cysteine + acetate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.47 37
-
-
 Triticum aestivum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.47 2
-
 inactivated above, after a few days Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 6.8
-
 phosphate buffer Triticum aestivum
2.5.1.47 8
-
 Tris-HCl buffer Triticum aestivum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.47 0.63
-
 5,5'-dithiobis(2-nitrobenzoic) acid pH 6.8, 37 C Triticum aestivum
2.5.1.47 1.32
-
 L-homoserine pH 6.8, 37 C Triticum aestivum
2.5.1.47 1.43
-
 N-ethylmaleimide pH 6.8, 37 C Triticum aestivum
2.5.1.47 1.75
-
 methionine pH 6.8, 37 C Triticum aestivum
2.5.1.47 2.27
-
 L-cysteine pH 6.8, 37 C Triticum aestivum
2.5.1.47 2.86
-
 p-chloromercuribenzoate pH 6.8, 37 C Triticum aestivum