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Literature summary extracted from

  • Murakoshi, I.; Ikegami, F.; Kaneko, M.
    Purification and properties of cysteine synthase from Spinacia oleracea (1985), Phytochemistry, 24, 1907-1911.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.022
-
 H2S pH 8.0, 30°C Spinacia oleracea
2.5.1.47 2.9
-
 O-acetyl-L-Ser pH 8.0, 30°C Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 32000
-
 2 * 32000, SDS-PAGE Spinacia oleracea
2.5.1.47 60000
-
 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 543
-
-
 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.5.1.47 0 C, potassium phosphate buffer, pH 8.0, 2-mercaptoethanol, EDTA, several months, no loss of activity Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 additional information beta-substituted alanines, low activity Spinacia oleracea ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + 2-propene-1-thiol 18% of activity with sulfide Spinacia oleracea S-allyl-L-cysteine + ?
-
 ?
2.5.1.47 O-acetyl-L-Ser + H2S
-
 Spinacia oleracea L-Cys + acetate
-
 ?
2.5.1.47 O-acetyl-L-Ser + mercaptoacetic acid 2.5% of activity with sulfide Spinacia oleracea S-carboxymethyl-L-cysteine
-
 ?
2.5.1.47 O-acetyl-L-Ser + methyl mercaptan 32% of activity with sulfide Spinacia oleracea S-methylcysteine + acetate
-
 ?
2.5.1.47 O-acetyl-L-serine
-
 Spinacia oleracea L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 32000, SDS-PAGE Spinacia oleracea
2.5.1.47 More amino acid composition Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 8.3
-
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate one per subunit Spinacia oleracea