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Literature summary extracted from

  • Ikegami, F.; Kaneko, M.; Kobori, M.; Murakoshi, I.
    Purification and characterization of cysteine synthase from Brassica juncea (1988), Phytochemistry, 27, 3379-3383.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.043
-
H2S pH 8.0, 30°C Brassica juncea
2.5.1.47 2.5
-
O-acetyl-L-Ser pH 8.0, 30°C Brassica juncea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 26000
-
2 * 26000, SDS-PAGE Brassica juncea
2.5.1.47 52000
-
gel filtration Brassica juncea

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Brassica juncea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
Brassica juncea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 leaf
-
Brassica juncea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 410
-
-
Brassica juncea

Storage Stability

EC Number Storage Stability Organism
2.5.1.47 0 C, potassium phosphate buffer, pH 8, mercaptoethanol, EDTA, three months, no loss of activity Brassica juncea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 additional information beta-substituted alanines, low activity Brassica juncea ?
-
?
2.5.1.47 O-acetyl-L-Ser + 2-propene-1-thiol 18% of activity with sulfide Brassica juncea S-allyl-L-cysteine + ?
-
?
2.5.1.47 O-acetyl-L-Ser + H2S
-
Brassica juncea L-Cys + acetate
-
?
2.5.1.47 O-acetyl-L-Ser + mercaptoacetic acid 6.1% of activity with sulfide Brassica juncea S-carboxymethyl-L-cysteine
-
?
2.5.1.47 O-acetyl-L-Ser + NaCN 12.3% of activity with sulfide Brassica juncea beta-cyanoalanine + sodium acetate
-
?
2.5.1.47 O-acetyl-L-serine
-
Brassica juncea L-cysteine + acetate
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 26000, SDS-PAGE Brassica juncea
2.5.1.47 More amino acid composition Brassica juncea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 8
-
-
Brassica juncea

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate one per subunit Brassica juncea