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Literature summary extracted from

  • Kuske, C.R.; Ticknor, L.O.; Guzman, E.; Gurley, L.R.; Valdez, J.G.; Thompson, M.E.; Jackson, P.J.
    Purification and characterization of O-acetylserine sulfhydrylase isoenzymes from Datura innoxia (1994), J. Biol. Chem., 269, 6223-6232.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.47 Sulfide above 200 mM Datura inoxia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 additional information
-
 additional information no Michaelis-Menten-kinetics Datura inoxia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 31200
-
 1 * 32000 + 1 * 31200, isoenzyme 2, SDS-PAGE Datura inoxia
2.5.1.47 32000
-
 2 * 32000, isoenzymes 1 and 3, SDS-PAGE Datura inoxia
2.5.1.47 32000
-
 1 * 32000 + 1 * 31200, isoenzyme 2, SDS-PAGE Datura inoxia
2.5.1.47 63000
-
 isoenzymes one and three, gel filtration Datura inoxia
2.5.1.47 86000
-
 isoenzyme 2, gel filtration Datura inoxia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + sulfide Datura inoxia involved in glutathione formation L-Cys + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Datura inoxia
-
 three isoenzymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47
-
 Datura inoxia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.47 cell suspension culture
-
 Datura inoxia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.47 880
-
 isoenzyme 1 and 2 Datura inoxia

Storage Stability

EC Number Storage Stability Organism
2.5.1.47 -70°C, Tris-HCl, pH 8.1, glycerol, 6 months, no loss of activity Datura inoxia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-Ser + sulfide involved in glutathione formation Datura inoxia L-Cys + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 32000, isoenzymes 1 and 3, SDS-PAGE Datura inoxia
2.5.1.47 dimer 1 * 32000 + 1 * 31200, isoenzyme 2, SDS-PAGE Datura inoxia
2.5.1.47 More amino acid composition Datura inoxia

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.47 42 58 all isoenzymes Datura inoxia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.47 7.6
-
 all isoenzymes Datura inoxia

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate responsible for O-acetylserine binding Datura inoxia