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Literature summary extracted from

  • Fujikura, K.; Zhang, Y.W.; Fujihashi, M.; Miki, K.; Koyama, T.
    Mutational analysis of allylic substrate binding site of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase (2003), Biochemistry, 42, 4035-4041.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.31 mutated enzymes are overproduced in Escherichia coli Micrococcus luteus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.31 D29A Km-value for farnesyl diphosphate is 9.2fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 5.8fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 1% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 D29A Km-value for farnesyl diphosphate is 9.2fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 5.8fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 1% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 E76Q Km-value for farnesyl diphosphate is 1.6fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 3.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.5fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 30% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 E76Q Km-value for farnesyl diphosphate is 1.6fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 3.6fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.5fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 30% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 E84Q Km-value for farnesyl diphosphate is 3.2fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.98fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 65% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 E84Q Km-value for farnesyl diphosphate is 3.2fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.98fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 65% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 G32R Km-value for farnesyl diphosphate is comparable to that of the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.9fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is comparable to that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 27% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 G32R Km-value for farnesyl diphosphate is comparable to that of the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.9fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is comparable to that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 27% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 G32R/R42G Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.4fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 58% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 G32R/R42G Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.4fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 1.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 58% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 R33A Km-value for farnesyl diphosphate is 40fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.2fold lower than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 3% of that of the wild-type enzyme. Different product distribution pattern compared to the wildf-type enzyme Micrococcus luteus
2.5.1.31 R33A Km-value for farnesyl diphosphate is 40fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.2fold lower than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 3% of that of the wild-type enzyme. Different product distribution pattern compared to the wildf-type enzyme Micrococcus luteus
2.5.1.31 R42G Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.8fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.1fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 13% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 R42G Km-value for farnesyl diphosphate is 1.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.8fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 3.1fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is 13% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 R80A Km-value for farnesyl diphosphate is 6.4fold higher than that for the wild-type enzyme. Km-value for (Z,E,E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 6.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is less than 1% of that of the wild-type enzyme Micrococcus luteus
2.5.1.31 R80A Km-value for farnesyl diphosphate is 6.4fold higher than that for the wild-type enzyme. Km-value for (2Z,6E,10E)-geranylgeranyl diphosphate is 1.3fold lower than that of the wild-type enzyme. Km-value for isopentenyl diphosphate is 6.3fold higher than that of the wild-type enzyme. The turnover-number for farnesyl diphosphate is less than 1% of that of the wild-type enzyme Micrococcus luteus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.31 SO42- competitive inhibitor with respect to (2E,6E)-farnesyl diphosphate; competitive inhibitor with respect to farnesyl diphosphate Micrococcus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.31 0.0023
-
farnesyl diphosphate mutant enzyme E76Q, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0023
-
(Z,E,E)-Geranylgeranyl diphosphate mutant enzyme E76Q, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0023
-
(2E,6E)-farnesyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0033
-
farnesyl diphosphate mutant enzyme G32R, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0033
-
(2E,6E)-farnesyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0036
-
farnesyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0036
-
(2E,6E)-farnesyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0043
-
(Z,E,E)-Geranylgeranyl diphosphate mutant enzyme G32R, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0043
-
(2Z,6E,10E)-geranylgeranyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0045
-
(Z,E,E)-Geranylgeranyl diphosphate mutant enzyme R42G, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0045
-
(2Z,6E,10E)-geranylgeranyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0049
-
farnesyl diphosphate mutant enzyme R42G, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0049
-
(2E,6E)-farnesyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.005
-
farnesyl diphosphate mutant enzyme G32R/R42G, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.005
-
(Z,E,E)-Geranylgeranyl diphosphate mutant enzyme D29A, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.005
-
(2Z,6E,10E)-geranylgeranyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.005
-
(2E,6E)-farnesyl diphosphate G32R/R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0059
-
isopentenyl diphosphate mutant enzyme G32R/R42G, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0059
-
(Z,E,E)-Geranylgeranyl diphosphate mutant enzyme G32R/R42G, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0059
-
(2Z,6E,10E)-geranylgeranyl diphosphate G32R-R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0059
-
isopentenyl diphosphate G32R/R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.006
-
isopentenyl diphosphate mutant enzyme G32R, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.006
-
(Z,E,E)-Geranylgeranyl diphosphate mutant enzyme E84Q, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.006
-
(2Z,6E,10E)-geranylgeranyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.006
-
isopentenyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0061
-
(Z,E,E)-Geranylgeranyl diphosphate mutant enzyme R80A, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0061
-
(2Z,6E,10E)-geranylgeranyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0078
-
isopentenyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0078
-
isopentenyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0082
-
(Z,E,E)-Geranylgeranyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0082
-
(2Z,6E,10E)-geranylgeranyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0088
-
(2E,6E,10E)-geranylgeranyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0095
-
(Z,E,E)-Geranylgeranyl diphosphate mutant enzyme R33A, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0095
-
(2Z,6E,10E)-geranylgeranyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0114
-
isopentenyl diphosphate mutant enzyme E76Q, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0114
-
farnesyl diphosphate mutant enzyme E84Q, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0114
-
isopentenyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0114
-
(2E,6E)-farnesyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.023
-
(2Z,6E,10E)-geranylgeranyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0231
-
farnesyl diphosphate mutant enzyme R80A, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0231
-
(2E,6E)-farnesyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0242
-
isopentenyl diphosphate mutant enzyme R42G, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0242
-
isopentenyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0307
-
isopentenyl diphosphate mutant enzyme E84Q, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0307
-
isopentenyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0332
-
farnesyl diphosphate mutant enzyme D29A, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0332
-
(2E,6E)-farnesyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0452
-
isopentenyl diphosphate mutant enzyme D29A, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0452
-
isopentenyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0491
-
isopentenyl diphosphate mutant enzyme R80A, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0491
-
isopentenyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.064
-
(2E,6E,10E)-geranylgeranyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.144
-
farnesyl diphosphate mutant enzyme R33A, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.144
-
(2E,6E)-farnesyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.31 additional information Micrococcus luteus catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate ?
-
?
2.5.1.31 additional information Micrococcus luteus B-P 26 catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.31 Micrococcus luteus
-
B-P 26
-
2.5.1.31 Micrococcus luteus O82827 B-P 26
-
2.5.1.31 Micrococcus luteus B-P 26
-
B-P 26
-
2.5.1.31 Micrococcus luteus B-P 26 O82827 B-P 26
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.31 (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
Micrococcus luteus 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
2.5.1.31 (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
Micrococcus luteus B-P 26 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
?
2.5.1.31 (2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
Micrococcus luteus ?
-
?
2.5.1.31 (2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
Micrococcus luteus B-P 26 ?
-
?
2.5.1.31 (2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
Micrococcus luteus ?
-
?
2.5.1.31 (2Z,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
Micrococcus luteus B-P 26 ?
-
?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate (E,E)-farnesyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
?
2.5.1.31 isopentenyl diphosphate + farnesyl diphosphate (E,E)-farnesyl diphosphate Micrococcus luteus B-P 26 C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
?
2.5.1.31 isopentenyl diphosphate + geranylgeranyl diphosphate (Z,E,E)-geranylgeranyl diphosphate Micrococcus luteus C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
?
2.5.1.31 isopentenyl diphosphate + geranylgeranyl diphosphate (Z,E,E)-geranylgeranyl diphosphate Micrococcus luteus B-P 26 C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
?
2.5.1.31 additional information catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate Micrococcus luteus ?
-
?
2.5.1.31 additional information catalyzes the sequential cis-condensation of isopentenyl diphosphate onto (2E,6E)-farnesyl diphosphate Micrococcus luteus B-P 26 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.31 undecaprenyl diphosphate synthase
-
Micrococcus luteus
2.5.1.31 UPP synthase
-
Micrococcus luteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.31 37
-
assay at Micrococcus luteus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.31 0.004
-
(2E,6E)-farnesyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.00579
-
(2Z,6E,10E)-geranylgeranyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0167
-
farnesyl diphosphate mutant enzyme D29A, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0167
-
(2E,6E)-farnesyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0258
-
(2Z,6E,10E)-geranylgeranyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0542
-
farnesyl diphosphate mutant enzyme R33A, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0542
-
(2E,6E)-farnesyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.0813
-
(2Z,6E,10E)-geranylgeranyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.216
-
farnesyl diphosphate mutant enzyme R42G, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.216
-
(2E,6E)-farnesyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.382
-
(2Z,6E,10E)-geranylgeranyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.462
-
farnesyl diphosphate mutant enzyme G32R, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.462
-
(2E,6E)-farnesyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.505
-
farnesyl diphosphate mutant enzyme E76Q, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.505
-
(2E,6E)-farnesyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.604
-
(2Z,6E,10E)-geranylgeranyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.608
-
(2Z,6E,10E)-geranylgeranyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.96
-
farnesyl diphosphate mutant enzyme G32R/R42G, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.96
-
(2E,6E)-farnesyl diphosphate G32R/R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 1.07
-
(2Z,6E,10E)-geranylgeranyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 1.09
-
farnesyl diphosphate mutant enzyme E84Q, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 1.09
-
(2E,6E)-farnesyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 1.11
-
(2Z,6E,10E)-geranylgeranyl diphosphate G32R/R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 1.66
-
farnesyl diphosphate wild-type enzyme, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 1.66
-
(2E,6E)-farnesyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 1.77
-
(2Z,6E,10E)-geranylgeranyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.31 7.5
-
assay at Micrococcus luteus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.31 17
-
SO42-
-
Micrococcus luteus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.31 0.25
-
(2E,6E)-farnesyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.713
-
(2E,6E)-farnesyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.777
-
(2E,6E)-farnesyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 0.949
-
(2Z,6E,10E)-geranylgeranyl diphosphate R80A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 5.2
-
(2Z,6E,10E)-geranylgeranyl diphosphate D29A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 8.65
-
(2Z,6E,10E)-geranylgeranyl diphosphate R33A mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 26.3
-
(2Z,6E,10E)-geranylgeranyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 77.9
-
(2E,6E)-farnesyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 84.8
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(2Z,6E,10E)-geranylgeranyl diphosphate R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 93.9
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(2E,6E)-farnesyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 141
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(2Z,6E,10E)-geranylgeranyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 178
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(2Z,6E,10E)-geranylgeranyl diphosphate E84Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 184
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(2E,6E)-farnesyl diphosphate G32R mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 188
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(2Z,6E,10E)-geranylgeranyl diphosphate G32R/R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 215.9
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(2Z,6E,10E)-geranylgeranyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 222
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(2E,6E)-farnesyl diphosphate G32R/R42G mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 262
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(2E,6E)-farnesyl diphosphate E76Q mutant, pH 7.5, 37°C Micrococcus luteus
2.5.1.31 461
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(2E,6E)-farnesyl diphosphate wild-type, pH 7.5, 37°C Micrococcus luteus