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Literature summary extracted from

  • Garbers, D.L.
    Demonstration of 5'-methylthioadenosine phosphorylase activity in various rat tissues. Some properties of the enzyme from rat lung (1978), Biochim. Biophys. Acta, 523, 82-93.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.28 putrescine no effect Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.28 2-Deoxyribose 1-phosphate
-
 Rattus norvegicus
2.4.2.28 adenine
-
 Rattus norvegicus
2.4.2.28 fructose 1-phosphate
-
 Rattus norvegicus
2.4.2.28 guanine
-
 Rattus norvegicus
2.4.2.28 L-methionine weak Rattus norvegicus
2.4.2.28 additional information no inhibition by EDTA, putrescine, cadaverine Rattus norvegicus
2.4.2.28 ribose 1-phosphate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.28 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.28 additional information no requirement for metal ion, e.g. Mg2+, Mn2+ or Ca2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.28 from lung Rattus norvegicus
2.4.2.28 partial Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.28 S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate phosphorolytic mechanism Rattus norvegicus
a
2.4.2.28 S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate equilibrium-ordered reaction, 5'-methylthioadenosine is the first substrate to bind and 5-methylthioribose 1-phosphate is the first product to be released Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.28 heart low activity Rattus norvegicus
-
2.4.2.28 kidney
-
 Rattus norvegicus
-
2.4.2.28 liver
-
 Rattus norvegicus
-
2.4.2.28 lung
-
 Rattus norvegicus
-
2.4.2.28 additional information wide tissue distribution Rattus norvegicus
-
2.4.2.28 spleen
-
 Rattus norvegicus
-
2.4.2.28 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.28 additional information
-
 kinetics Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.2.28 -70°C, stable for at least 4 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 5'-methylthioadenosine + phosphate
-
 Rattus norvegicus adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.28 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.28 7.4 8
-
 Rattus norvegicus