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Literature summary extracted from

  • Cacciapuoti, G.; Porcelli, M.; Bertoldo, C.; De Rosa, M.; Zappia, V.
    Purification and characterization of extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Purine nucleoside phosphorylase activity and evidence for intersubunit disulfide bonds (1994), J. Biol. Chem., 269, 24762-24769.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.28 no loss of activity after 24 h at room temperature in presence of 9 M urea, 4 M guanidine hydrochloride, 0.075% SDS, 50% methanol, 50% ethanol, 50% dimethylformamide, 1 M NaCl, and 1% Triton X-100 Saccharolobus solfataricus
2.4.2.28 no loss of activity after treatment with thermolysin, trypsin, and chymotrypsin for 24 h at 37°C Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.28 iodoacetate incorporation of 12 mol iodoactate per mol of enzyme Saccharolobus solfataricus
2.4.2.28 additional information activity is not affected by alkylating, mercaptide-forming and oxidizing thiol reagents; no inhibition by S-adenosyl-L-methionine Saccharolobus solfataricus
2.4.2.28 S-adenosyl-L-homocysteine no inhibition Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.28 0.024
-
 5'-methylthioadenosine
-
 Saccharolobus solfataricus
2.4.2.28 0.123
-
 phosphate
-
 Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.28 cytosol
-
 Saccharolobus solfataricus 5829
-
2.4.2.28 cytosol exclusively Saccharolobus solfataricus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.28 27000
-
 6 * 27000, SDS-PAGE Saccharolobus solfataricus
2.4.2.28 160000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.28 5'-methylthioadenosine + phosphate Saccharolobus solfataricus
-
 adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 adenosine + phosphate Saccharolobus solfataricus
-
 adenine + D-ribose 1-phosphate
-
 ?
2.4.2.28 guanosine + phosphate Saccharolobus solfataricus
-
 guanine + D-ribose 1-phosphate
-
 ?
2.4.2.28 inosine + phosphate Saccharolobus solfataricus
-
 hypoxanthine + D-ribose 1-phosphate
-
 ?
2.4.2.28 additional information Saccharolobus solfataricus involved in the catabolism of 5'-methylthioadenosine, adenosine, guanosine and inosine ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.4.2.28 1-propanol no loss of activity after 24 h at room temperature in presence of 50% methanol, loss of 90% activity at 90°C after 1 h Saccharolobus solfataricus
2.4.2.28 acetonitrile loss of 89% activity at 70°C after 1 h Saccharolobus solfataricus
2.4.2.28 dimethylformamide no loss of activity after 24 h at room temperature in presence of 50% dimethylformamide, loss of 21% activity at 90°C after 1 h Saccharolobus solfataricus
2.4.2.28 Ethanol no loss of activity after 24 h at room temperature in presence of 50% ethanol, loss of 88% activity at 90°C after 1 h Saccharolobus solfataricus
2.4.2.28 Ethanol 50%, 24 h at room temperature, no loss of activity Saccharolobus solfataricus
2.4.2.28 guanidine-HCl 4 M, 24 h at room temperature, no loss of activity Saccharolobus solfataricus
2.4.2.28 Methanol no loss of activity after 24 h at room temperature in presence of 50% methanol, loss of 80% activity at 90°C after 1 h Saccharolobus solfataricus
2.4.2.28 Methanol 50%, 24 h at room temperature, no loss of activity Saccharolobus solfataricus
2.4.2.28 N,N-dimethylformamide 50%, 24 h at room temperature, no loss of activity Saccharolobus solfataricus
2.4.2.28 SDS 0.075%, 24 h at room temperature, no loss of activity. After 60 min of incubation in 2% SDS the enzyme remains fully active at 70°C and retains 20% residual activity at 90°C. Complete inactivation after 5 min at 100°C in 0.5% SDS Saccharolobus solfataricus
2.4.2.28 tetrahydrofuran loss of 76% activity at 70°C after 30 min, complete loss of activity after 1 h at 70°C Saccharolobus solfataricus
2.4.2.28 Triton X-100 9 M, 24 h at room temperature, no loss of activity Saccharolobus solfataricus
2.4.2.28 urea 9 M, 24 h at room temperature, no loss of activity Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Saccharolobus solfataricus
-
-
-
2.4.2.28 Saccharolobus solfataricus P50389
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.28
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.28 2.116
-
-
 Saccharolobus solfataricus
2.4.2.28 2.12
-
 purified enzyme Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
2.4.2.28 -20°C, 10 mM Tris-HC1, pH 7.4, containing 1 mM EDTA, stable for at least 1 year Saccharolobus solfataricus
2.4.2.28 -20°C, purified enzyme, Tris-HCl 10 mM, pH 7.4, 1 mM EDTA, stable at least 1 year Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 5'-methylthioadenosine + phosphate
-
 Saccharolobus solfataricus adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 adenosine + phosphate
-
 Saccharolobus solfataricus adenine + D-ribose 1-phosphate
-
 ?
2.4.2.28 guanosine + phosphate
-
 Saccharolobus solfataricus guanine + D-ribose 1-phosphate
-
 ?
2.4.2.28 inosine + phosphate
-
 Saccharolobus solfataricus hypoxanthine + D-ribose 1-phosphate
-
 ?
2.4.2.28 inosine + phosphate most effective substrate Saccharolobus solfataricus hypoxanthine + D-ribose 1-phosphate
-
 ?
2.4.2.28 additional information substrate specificity Saccharolobus solfataricus ?
-
 ?
2.4.2.28 additional information no activity with S-adenosyl-L-methionine Saccharolobus solfataricus ?
-
 ?
2.4.2.28 additional information no activity with S-adenosylhomocysteine Saccharolobus solfataricus ?
-
 ?
2.4.2.28 additional information involved in the catabolism of 5'-methylthioadenosine, adenosine, guanosine and inosine Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.28 hexamer 6 * 27000, SDS-PAGE Saccharolobus solfataricus
2.4.2.28 More secondary structure by circular dichroism spectra Saccharolobus solfataricus
2.4.2.28 More presence of 6 disulfide bonds, organisation in 2 trimers Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.28 5'-methylthioadenosine phosphorylase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.28 70
-
 assay at Saccharolobus solfataricus
2.4.2.28 120
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.28 30 150
-
 Saccharolobus solfataricus
2.4.2.28 90 130 90°C: about 50% of maximal activity, 130°C: about 70% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.28 100
-
 stable for at least 2 h Saccharolobus solfataricus
2.4.2.28 120
-
 2 h, stable Saccharolobus solfataricus
2.4.2.28 130
-
 half-life: 15 min Saccharolobus solfataricus
2.4.2.28 130
-
 half-inactivation time: 15 min Saccharolobus solfataricus
2.4.2.28 132
-
 10 min, melting temperature Saccharolobus solfataricus
2.4.2.28 140
-
 half-life: 5 min Saccharolobus solfataricus
2.4.2.28 140
-
 half-inactivation time: 5 min Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.28 additional information
-
 pI: 5.7 Saccharolobus solfataricus
2.4.2.28 7.4
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.28 5 10
-
 Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.2.28 Saccharolobus solfataricus isoelectric focusing on polyacrylamide gel in the pH ranges 3-10 and 4.5-6.0
-
 5.7