EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.28 | expression at a high level in Escherichia coli | Saccharolobus solfataricus |
2.4.2.28 | overexpression in Escherichia coli strain RB791, amino acid determination, incorrect positioning of disulfide bonds, the recombinant enzyme is less thermostable and thermophilic than the native enzyme | Saccharolobus solfataricus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.28 | guanidine hydrochloride | only recombinant enzyme | Saccharolobus solfataricus | |
2.4.2.28 | iodoacetamide | only recombinant enzyme | Saccharolobus solfataricus | |
2.4.2.28 | iodoacetate | only recombinant enzyme | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.28 | 0.024 | - |
5'-methylthioadenosine | recombinant enzyme | Saccharolobus solfataricus | |
2.4.2.28 | 0.024 | - |
5'-methylthioadenosine | wild-type and recombinant enzyme | Saccharolobus solfataricus | |
2.4.2.28 | 0.125 | - |
phosphate | recombinant enzyme | Saccharolobus solfataricus | |
2.4.2.28 | 0.125 | - |
phosphate | wild-type and recombinant enzyme | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.28 | 27000 | - |
6 * 27000, recombinant enzyme, SDS-PAGE | Saccharolobus solfataricus |
2.4.2.28 | 160000 | - |
gel filtration | Saccharolobus solfataricus |
2.4.2.28 | 160000 | - |
recombinant enzyme, gel filtration | Saccharolobus solfataricus |
2.4.2.28 | 160000 | - |
recombinant enzyme | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.28 | Saccharolobus solfataricus | - |
- |
- |
2.4.2.28 | Saccharolobus solfataricus | P50389 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.28 | recombinant enzyme | Saccharolobus solfataricus |
2.4.2.28 | recombinant from Escherichia coli | Saccharolobus solfataricus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.28 | 2.115 | - |
- |
Saccharolobus solfataricus |
2.4.2.28 | 2.12 | - |
purified recombinant enzyme | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.28 | 5'-methylthioadenosine + phosphate | - |
Saccharolobus solfataricus | adenine + 5-methylthio-D-ribose 1-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.28 | hexamer | 6 * 27000, recombinant enzyme, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.28 | 5'-methylthioadenosine phosphorylase | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.28 | 70 | - |
assay at | Saccharolobus solfataricus |
2.4.2.28 | 100 | - |
recombinant enzyme | Saccharolobus solfataricus |
2.4.2.28 | 120 | - |
wild-type enzyme | Saccharolobus solfataricus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.28 | 30 | 120 | recombinant enzyme | Saccharolobus solfataricus |
2.4.2.28 | 60 | 120 | 60°C: about 40% of maximal activity, 120°C: about 40% of maximal activity, recombinant enzyme | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.28 | additional information | - |
the recombinant enzyme, expressed in Escherichia coli, is less thermostable and thermophilic than the native enzyme due to incorrect positioning of disulfide bonds | Saccharolobus solfataricus |
2.4.2.28 | additional information | - |
the recombinant 5'-methylthioadenosine phosphorylase is less thermophilic and thermostable than the Sulfolobus solfataricus enzyme, since an incorrect positioning of disulfide bonds within the molecule generates structures less stable to thermal unfolding | Saccharolobus solfataricus |
2.4.2.28 | 70 | - |
recombinant enzyme, 0.8 M DTT, stable | Saccharolobus solfataricus |
2.4.2.28 | 90 | - |
recombinant enzyme, 0.8 M DTT, 2 h, loss of 38% activity | Saccharolobus solfataricus |
2.4.2.28 | 100 | - |
recombinant enzyme, 1 h, 85% remaining activity | Saccharolobus solfataricus |
2.4.2.28 | 100 | - |
1 h, 15% loss of activity of recombinant enzyme, wild-type enzyme remains stable | Saccharolobus solfataricus |
2.4.2.28 | 111 | - |
recombinant enzyme, melting temperature | Saccharolobus solfataricus |
2.4.2.28 | 111 | - |
melting temperature of recombinant enzyme | Saccharolobus solfataricus |
2.4.2.28 | 118 | - |
recombinant enzyme in presence of 100 mM phosphate, melting temperature | Saccharolobus solfataricus |