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Literature summary extracted from

  • Appleby, T.C.; Mathews, I.I.; Porcelli, M.; Cacciapuoti, G.; Ealick, S.E.
    Three-dimensional structure of a hyperthermophilic 5'-deoxy-5'-methylthioadenosine phosphorylase from Sulfolobus solfataricus (2001), J. Biol. Chem., 276, 39232-39242.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.28 crystals are grown at either room temperature or 18°C using the hanging drop vapor diffusion technique. Determination of the structure of 5'-deoxy-5'-methylthioadenosine phosphorylase alone, as ternary complexes with sulfate plus substrates 5'-deoxy-5'-methylthioadenosine, adenosine, or guanosine, or with the noncleavable substrate analog formycin B and as binary complexes with phosphate or sulfate alone. The structure of unliganded SsMTAP is refined at 2.5 A resolution and the structures of the complexes are refined at resolutions ranging from 1.6 A to 2.0 A Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Saccharolobus solfataricus
-
-
-
2.4.2.28 Saccharolobus solfataricus P50389
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.28 recombinant from Escherichia coli Saccharolobus solfataricus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.28 S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate catalytic mechanism Saccharolobus solfataricus
a
2.4.2.28 S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate active and substrate-binding sites, three-dimensional structures Saccharolobus solfataricus
a
2.4.2.28 S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate subunit structure, model Saccharolobus solfataricus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 5'-deoxy-5'-methylthioadenosine + phosphate substrate-induced conformational change involving Glu163, which is located at the interface between subunits and swings in toward the active site upon nucleoside binding Saccharolobus solfataricus adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
2.4.2.28 5'-deoxy-5'-methylthioadenosine + phosphate the transition state is stabilized in different ways for 6-amino versus 6-oxo substrates Saccharolobus solfataricus adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.28 hexamer
-
 Saccharolobus solfataricus
2.4.2.28 More enzyme is formed by a trimer of dimers with three symmetric intersubunit disulfide bonds linking the dimers to one another, each monomer contains one active site, which is located near a dimer interface Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.28 5'-deoxy-5'-methylthioadenosine phosphorylase
-
 Saccharolobus solfataricus
2.4.2.28 SsMTAP
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.28 120
-
-
 Saccharolobus solfataricus
2.4.2.28 120
-
 recombinant enzyme Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.28 additional information
-
 disulfide linkages play a key role in thermal stability Saccharolobus solfataricus
2.4.2.28 100
-
 2 h, stable Saccharolobus solfataricus
2.4.2.28 110
-
 recombinant enzyme, 2 h, stable Saccharolobus solfataricus
2.4.2.28 120
-
-
 Saccharolobus solfataricus