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Literature summary extracted from

  • Wickus, G.G.; Strominger, J.L.
    Partial purification and properties of the pyruvate-uridine diphospho-N-acetylglucosamine transferase from Staphylococcus epidermidis (1973), J. Bacteriol., 113, 287-290.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.5.1.7 EDTA, DTT and dithioerythritol stabilize Staphylococcus epidermidis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.7 5,5'-dithiobis(2-nitrobenzoic acid) complete inhibition at 0.01 M; i.e. DTNB Staphylococcus epidermidis
2.5.1.7 N-ethylmaleimide complete inhibition at 0.2 M Staphylococcus epidermidis
2.5.1.7 UDP-N-acetylmuramic acid
-
 Staphylococcus epidermidis
2.5.1.7 UDP-N-acetylmuramic acid-L-Ala-D-Glu no inhibition Staphylococcus epidermidis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.7 Staphylococcus epidermidis
-
-
-
2.5.1.7 Staphylococcus epidermidis Texas 26
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.7 partial Staphylococcus epidermidis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.7 0.2
-
 partially purified enzyme Staphylococcus epidermidis

Storage Stability

EC Number Storage Stability Organism
2.5.1.7 -15°C, crude enzyme preparation, in presence of 4 mM DTT, loss of 84% activity within 2 weeks Staphylococcus epidermidis
2.5.1.7 -196°C, 4 mM DTT, stable for up to 6 weeks, partially purified enzyme Staphylococcus epidermidis
2.5.1.7 -196°C, slightly increased activity after storage of 2 weeks Staphylococcus epidermidis
2.5.1.7 -70°C, 4 mM DTT, 22% loss of activity in 2 weeks, partially purified enzyme Staphylococcus epidermidis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Staphylococcus epidermidis phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Staphylococcus epidermidis Texas 26 phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.7 37
-
 assay at Staphylococcus epidermidis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.7 7.4
-
-
 Staphylococcus epidermidis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.7 6.5 8.4
-
 Staphylococcus epidermidis