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Literature summary extracted from

  • Sagami, I.; Fujii, H.; Koyama, T.; Ogura, K.
    Heptaprenylpyrophosphate synthetase from Bacillus subtilis (1985), Methods Enzymol., 110, 199-205.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.30 0.0083
-
 geranylgeranyl diphosphate pH 7.5, 37°C Bacillus subtilis
2.5.1.30 0.0128
-
 isopentenyl diphosphate pH 7.5, 37°C Bacillus subtilis
2.5.1.30 0.0133
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 37°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.30 Mg2+ absolute requirement Bacillus subtilis
2.5.1.30 Mn2+ 30% of the activation obtained with Mg2+ Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.30 30000
-
 molecular weight of enzyme component I and enzyme component II is both about 30000 Da, gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.30 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate Bacillus subtilis synthesis of all-trans-heptaprenyl diphosphate, which is precursor for the side chain of menaquinone-7 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.30 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate Bacillus subtilis PCI-219 synthesis of all-trans-heptaprenyl diphosphate, which is precursor for the side chain of menaquinone-7 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.30 Bacillus subtilis P31112 and P31114 P31112: subunit 1, P31114: subunit 2; PCI-219
-
2.5.1.30 Bacillus subtilis PCI-219 P31112 and P31114 P31112: subunit 1, P31114: subunit 2; PCI-219
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.30 separation of component I and component II Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.30 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate
-
 Bacillus subtilis 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.30 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate synthesis of all-trans-heptaprenyl diphosphate, which is precursor for the side chain of menaquinone-7 Bacillus subtilis 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.30 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate
-
 Bacillus subtilis PCI-219 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.30 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate synthesis of all-trans-heptaprenyl diphosphate, which is precursor for the side chain of menaquinone-7 Bacillus subtilis PCI-219 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.30 geranylgeranyl diphosphate + 3 isopentenyl diphosphate
-
 Bacillus subtilis 3 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.30 geranylgeranyl diphosphate + 3 isopentenyl diphosphate
-
 Bacillus subtilis PCI-219 3 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.30 additional information neither dimethylallyl diphosphate nor geranyl diphosphate is active as primer Bacillus subtilis ?
-
 ?
2.5.1.30 additional information neither dimethylallyl diphosphate nor geranyl diphosphate is active as primer Bacillus subtilis PCI-219 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.30 ? molecular weight of enzyme component I and enzyme component II is both about 30000 Da, gel filtration Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.5.1.30 heptaprenylpyrophosphate synthetase
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.30 50
-
 component II is inactivated almost completely within 5 min, component II does not lose activity within 30 min Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.30 6.5 7
-
 Bacillus subtilis