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Literature summary extracted from

  • Tani, Y.; Ukita, M.; Ogata, K.
    Studies on vitamin b6 metabolism in microorganisms. Part X. Further purification and characterization of pyridoxamine 5'-phosphate-alpha-ketoglutarate transaminase from Clostridium kainantoi (1972), Agric. Biol. Chem., 36, 181-188.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.54 4-aminobutyrate stimulation, 177% of non-stimulated activity Clostridium butyricum
2.6.1.54 D-asparagine stimulation, 112% of non-stimulated activity Clostridium butyricum
2.6.1.54 D-cysteine stimulation, 135% of non-stimulated activity Clostridium butyricum
2.6.1.54 D-methionine stimulation, 127% of non-stimulated activity Clostridium butyricum
2.6.1.54 DL-Ornithine stimulation, 112% of non-stimulated activity Clostridium butyricum
2.6.1.54 glycine stimulation, 137% of non-stimulated activity Clostridium butyricum
2.6.1.54 L-asparagine stimulation, 112% of non-stimulated activity Clostridium butyricum
2.6.1.54 L-glutamate stimulation, 166% of non-stimulated activity Clostridium butyricum
2.6.1.54 L-lysine stimulation, 105% of non-stimulated activity Clostridium butyricum
2.6.1.54 L-methionine stimulation, 114% of non-stimulated activity Clostridium butyricum
2.6.1.54 L-ornithine stimulation, 112% of non-stimulated activity Clostridium butyricum
2.6.1.54 additional information stimulating effect of amino compounds may be caused by disappearance of the inhibitory product of the reaction, pyridoxal 5'-phosphate, forming the corresponding Schiff base Clostridium butyricum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.54 D-glutamate slight inhibition Clostridium butyricum
2.6.1.54 diisopropyl phosphofluoride 0.3 mM, complete inhibition Clostridium butyricum
2.6.1.54 L-cysteine about 35% inhibition Clostridium butyricum
2.6.1.54 additional information not inhibited by sulfhydryl compounds, e.g. PCMB, phenylmercuric acetate and mercuric chloride Clostridium butyricum
2.6.1.54 pyridoxal 5'-phosphate product inhibition Clostridium butyricum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.54 0.004
-
 pyridoxamine 5'-phosphate pH 8.0, 37°C Clostridium butyricum
2.6.1.54 0.01
-
 pyridoxal 5'-phosphate pH 8.0, 37°C Clostridium butyricum
2.6.1.54 0.015
-
 2-oxoglutarate cosubstrate pyridoxamine 5'-phosphate, pH 8.0, 37°C Clostridium butyricum
2.6.1.54 9
-
 D-glutamate pH 8.0, 37°C Clostridium butyricum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.6.1.54 additional information metal ions do not accelerate the reaction Clostridium butyricum

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.54 Clostridium butyricum
-
 IFO 3353
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.54 ammonium sulfate, protamine treatment, DEAE-cellulose, hydroxylapatite, DEAE-Sephadex, Sephadex G-200, partial purification Clostridium butyricum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.54 0.281
-
-
 Clostridium butyricum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.54 4-aminobutanoate + 2-oxoglutarate about 8% of activity compared to pyridoxamine 5'-phosphate Clostridium butyricum 4-oxobutanoate + D-glutamate
-
 ?
2.6.1.54 pyridoxamine + 2-oxoglutarate about 25% of activity compared to pyridoxamine 5'-phosphate Clostridium butyricum pyridoxal + D-glutamate
-
 ?
2.6.1.54 pyridoxamine 5'-phosphate + 2-oxoglutarate
-
 Clostridium butyricum pyridoxal 5'-phosphate + D-glutamate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.54 37
-
 assay at Clostridium butyricum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.54 8
-
-
 Clostridium butyricum