EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.32 | 0.0027 | - |
prephytoene diphosphate | pH 7.6, 30°C | Capsicum annuum | |
2.5.1.32 | 0.003 | - |
geranylgeranyl diphosphate | pH 7.6, 30°C | Capsicum annuum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.5.1.32 | chromoplast | stroma | Capsicum annuum | 9509 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.32 | Mn2+ | absolute requirement for Mn2+, half-maximal activity at 0.2 mM. No other divalent cation can substitute efficiently for Mn2+ | Capsicum annuum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.32 | 47500 | - |
1 * 47500, SDS-PAGE | Capsicum annuum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.32 | Capsicum annuum | - |
a single polypeptide catalyzes the 2-step reaction from geranylgeranyl diphosphate to phytoene | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.32 | - |
Capsicum annuum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.32 | fruit | - |
Capsicum annuum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.32 | 0.067 | - |
- |
Capsicum annuum |
EC Number | Storage Stability | Organism |
---|---|---|
2.5.1.32 | -20°C, 50 mM Tris-HCl buffer, pH 7.6, 2 mM dithiothreitol, 0.3 M NaCl, 30% glycerol, stable for 1 year | Capsicum annuum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.32 | geranylgeranyl diphosphate + geranylgeranyl diphosphate | the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene | Capsicum annuum | prephytoene diphosphate + diphosphate | - |
? | |
2.5.1.32 | prephytoene diphosphate | the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene | Capsicum annuum | phytoene + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.32 | monomer | 1 * 47500, SDS-PAGE | Capsicum annuum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.32 | 7.6 | - |
- |
Capsicum annuum |