Literature summary extracted from

Misawa, N.; Truesdale, M.R.; Sandmann, G.; Fraser, P.D.; Bird, C.; Schuch, W.; Bramley, P.M.
Expression of a tomato cDNA coding for phytoene synthase in Escherichia coli, phytoene formation in vivo and in vitro, and functional analysis of the various truncated gene products (1994), J. Biochem., 116, 980-985.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.5.1.32	full length and truncated cDNA expression constructs of the phytoene synthase gene from tomato ligated into a pUC8 cloning vector, expression in Escherichia coli carrying Erwinia uredovora geranylgeranyl diphosphate synthase gene	Solanum lycopersicum

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.5.1.32	additional information	the size of the transit peptide of phytoene synthase from ripe fruit is approximatyle 9000 Da, corresponding to about 80 amino acid residues. Removal of further N-terminal amino acids up to 114 from the enzyme, to yield a protein of apparent molecular mass 3400 Da, increases its catalytic activity in Escherichia coli	Solanum lycopersicum

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.5.1.32	Solanum lycopersicum	-	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
2.5.1.32	proteolytic modification	the size of the transit peptide of phytoene synthase from ripe fruit is approximatyle 9000 Da, corresponding to about 80 amino acid residues	Solanum lycopersicum

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.5.1.32	fruit	-	Solanum lycopersicum	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.5.1.32	geranylgeranyl diphosphate + geranylgeranyl diphosphate	-	Solanum lycopersicum	prephytoene diphosphate + diphosphate	-	?

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Release 2023.1 (January 2023)