Literature summary extracted from
Iwata-Reuyl, D.; Math, S.K.; Desai, S.B.; Poulter, C.D.
Bacterial phytoene synthase: molecular cloning, expression, and characterization of Erwinia herbicola phytoene synthase (2003), Biochemistry, 42, 3359-3365.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.5.1.32 |
Tween 80 |
detergent required, maximal activity at 0.08% |
Pantoea agglomerans |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.5.1.32 |
expression in Escherichia coli |
Pantoea agglomerans |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.5.1.32 |
additional information |
to facilitate purification of the recombinant enzyme, the structural gene for Psase is modified by site-directed mutagenesis to incorporate a C-terminal Glu-Glu-Phe tripeptide to allow purification by immunoaffinity chromatography on an immobilized monoclonal anti-alpha-tubulin antibody YL1/2 column |
Pantoea agglomerans |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
2.5.1.32 |
soluble |
upon induction the recombinant enzyme constitutes 5-10% of the total soluble protein |
Pantoea agglomerans |
- |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.5.1.32 |
Mn2+ |
required |
Pantoea agglomerans |
|
2.5.1.32 |
Mn2+ |
maximal activity at 0.25 mM |
Pantoea agglomerans |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.5.1.32 |
34500 |
- |
x * 34500, recombinant enzyme, SDS-PAGE |
Pantoea agglomerans |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.5.1.32 |
geranylgeranyl diphosphate + geranylgeranyl diphosphate |
Pantoea agglomerans |
first pathway-specific step in carotenoid biosynthesis |
prephytoene diphosphate + diphosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.5.1.32 |
Pantoea agglomerans |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.5.1.32 |
to facilitate purification of the recombinant enzyme, the structural gene for Psase is modified by site-directed mutagenesis to incorporate a C-terminal Glu-Glu-Phe tripeptide to allow purification by immunoaffinity chromatography on an immobilized monoclonal anti-alpha-tubulin antibody YL1/2 column |
Pantoea agglomerans |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.5.1.32 |
flash frozen in liquid N2 and stored at -80°C, stable for at least 6 months |
Pantoea agglomerans |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.5.1.32 |
geranylgeranyl diphosphate + geranylgeranyl diphosphate |
the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene |
Pantoea agglomerans |
prephytoene diphosphate + diphosphate |
- |
? |
|
2.5.1.32 |
geranylgeranyl diphosphate + geranylgeranyl diphosphate |
first pathway-specific step in carotenoid biosynthesis |
Pantoea agglomerans |
prephytoene diphosphate + diphosphate |
- |
? |
|
2.5.1.32 |
prephytoene diphosphate |
the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene |
Pantoea agglomerans |
phytoene + diphosphate |
15,15'-Z-phytoene is the sole product |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.5.1.32 |
? |
x * 34500, recombinant enzyme, SDS-PAGE |
Pantoea agglomerans |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.5.1.32 |
8.2 |
- |
- |
Pantoea agglomerans |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
2.5.1.32 |
6.8 |
8.8 |
about 70% of maximal activity at pH 6.8 and pH 8.8 |
Pantoea agglomerans |