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Literature summary extracted from

  • Fraser, P.D.; Schuch, W.; Bramley, P.M.
    Phytoene synthase from tomato (Lycopersicon esculentum) chloroplasts - partial purification and biochemical properties (2000), Planta, 211, 361-369.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.32 ATP Mn2+ and ATP are essential for catalytic activity, the effect is stoichiometric from 0.5-2 mM. Km for ATP is 2.0 mM Solanum lycopersicum
2.5.1.32 Triton X-100 0.1 w/v, 5fold stimulation Solanum lycopersicum
2.5.1.32 Tween 60 0.1 w/v, 5fold stimulation Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.32 ADP
-
 Solanum lycopersicum
2.5.1.32 AMP
-
 Solanum lycopersicum
2.5.1.32 beta-carotene
-
 Solanum lycopersicum
2.5.1.32 Chlorophyll
-
 Solanum lycopersicum
2.5.1.32 GTP
-
 Solanum lycopersicum
2.5.1.32 Mn2+ above 2 mM Solanum lycopersicum
2.5.1.32 NADP+
-
 Solanum lycopersicum
2.5.1.32 NADPH
-
 Solanum lycopersicum
2.5.1.32 phosphate
-
 Solanum lycopersicum
2.5.1.32 squalestatin
-
 Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.32 0.005
-
 geranylgeranyl diphosphate pH 8.0, 28°C, enzyme from chloroplast Solanum lycopersicum
2.5.1.32 0.01
-
 geranylgeranyl diphosphate pH 8.0, 28°C, enzyme from chromoplast Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.32 membrane membrane-associated. Treatment with high ionic strength buffer or mild non-ionic detergent is required for solubilization Solanum lycopersicum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.32 Mn2+ Mn2+ and ATP in combination are essential for catalytic activity, the effect is stoichiometric from 0.5-2.0 mM. Km for Mn2+ is 0.4 mM Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.32 43000
-
 native PAGE Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.32 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.32 partial Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.32 fruit
-
 Solanum lycopersicum
-
2.5.1.32 leaf low activity in young leaf Solanum lycopersicum
-

Storage Stability

EC Number Storage Stability Organism
2.5.1.32 -70°C, 50 mM Tris, pH 7.5, 10% v/v glycerol, 80% of the activity retained Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.32 geranylgeranyl diphosphate + geranylgeranyl diphosphate
-
 Solanum lycopersicum prephytoene diphosphate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.32 More the enzyme is functional in a monomeric state, under optimal native conditions it is associated with a large protein complex, at least 200000 Da, which contains other terpenoid enzymes Solanum lycopersicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.32 4
-
 10 h, stable Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.32 6.5
-
 enzyme from chloroplast Solanum lycopersicum
2.5.1.32 7.5
-
 enzyme from chromoplast Solanum lycopersicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.32 0.000007
-
 squalestatin pH 8.0, 28°C, chloroplast enzyme Solanum lycopersicum
2.5.1.32 0.000009
-
 squalestatin pH 8.0, 28°C, chromoplast enzyme Solanum lycopersicum
2.5.1.32 0.0002
-
 NADP+ pH 8.0, 28°C, chloroplast enzyme and chromoplast enzyme Solanum lycopersicum
2.5.1.32 0.1
-
 NADPH pH 8.0, 28°C, chloroplast enzyme Solanum lycopersicum
2.5.1.32 0.2
-
 phosphate pH 8.0, 28°C, chloroplast enzyme and chromoplast enzyme Solanum lycopersicum
2.5.1.32 0.4
-
 NADPH pH 8.0, 28°C, chromoplast enzyme Solanum lycopersicum
2.5.1.32 0.5
-
 phosphate pH 8.0, 28°C, chromoplast enzyme Solanum lycopersicum
2.5.1.32 1
-
 AMP pH 8.0, 28°C, chloroplast enzyme Solanum lycopersicum
2.5.1.32 3
-
 ADP pH 8.0, 28°C, chloroplast enzyme Solanum lycopersicum
2.5.1.32 4.3
-
 GTP pH 8.0, 28°C, chloroplast enzyme Solanum lycopersicum
2.5.1.32 4.3
-
 ADP pH 8.0, 28°C, chromoplast enzyme Solanum lycopersicum