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Literature summary extracted from

  • Vos, S.; de Jersey, J.; Martin, J.L.
    Crystal structure of Escherichia coli xanthine phosphoribosyltransferase (1997), Biochemistry, 36, 4125-4134.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.22 hanging drop vapor diffusion method Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.22 0.0043
-
 guanine
-
 Escherichia coli
2.4.2.22 0.0305
-
 xanthine
-
 Escherichia coli
2.4.2.22 0.0908
-
 hypoxanthine
-
 Escherichia coli
2.4.2.22 0.139
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.22 Mg2+ magnesium and sulfate can bind together in the active site, in the absence of other products or substrates. The magnesium interacts with a highly conserved aspartate residue Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.22 Escherichia coli P0A9M5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.22 5-phospho-alpha-D-ribose 1-diphosphate + guanine
-
 Escherichia coli GMP + diphosphate
-
 ?
2.4.2.22 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine
-
 Escherichia coli IMP + diphosphate
-
 ?
2.4.2.22 5-phospho-alpha-D-ribose 1-diphosphate + xanthine
-
 Escherichia coli 9-(5-phospho-beta-D-ribosyl)xanthine + diphosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.22 54.8
-
 hypoxanthine
-
 Escherichia coli
2.4.2.22 112
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Escherichia coli
2.4.2.22 112
-
 guanine
-
 Escherichia coli
2.4.2.22 150
-
 xanthine
-
 Escherichia coli