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Literature summary extracted from

  • Nagy, M.; Ribet, A.M.
    Purification and comparative study of adenine and guanine phosphoribosyltransferases from Schizosaccharomyces pombe (1977), Eur. J. Biochem., 77, 77-85.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.7 ADP
-
Schizosaccharomyces pombe
2.4.2.7 AMP
-
Schizosaccharomyces pombe
2.4.2.7 ATP
-
Schizosaccharomyces pombe
2.4.2.7 CTP
-
Schizosaccharomyces pombe
2.4.2.7 dAMP
-
Schizosaccharomyces pombe
2.4.2.7 dATP
-
Schizosaccharomyces pombe
2.4.2.7 diphosphate at 20 mM Schizosaccharomyces pombe
2.4.2.7 GDP slightly at 5 mM Schizosaccharomyces pombe
2.4.2.7 GMP slightly at 5 mM Schizosaccharomyces pombe
2.4.2.7 GTP slightly at 5 mM Schizosaccharomyces pombe
2.4.2.7 Mg2+ inhibitory effects are noncompetitive against 5-phosphoribose 1-diphosphate Schizosaccharomyces pombe
2.4.2.7 additional information mechanism of product inhibition; no inhibition by GMP, XMP, UMP, CMP, and CDP Schizosaccharomyces pombe
2.4.2.7 UDP slightly Schizosaccharomyces pombe
2.4.2.8 AMP
-
Schizosaccharomyces pombe
2.4.2.8 CDP
-
Schizosaccharomyces pombe
2.4.2.8 diphosphate
-
Schizosaccharomyces pombe
2.4.2.8 GDP
-
Schizosaccharomyces pombe
2.4.2.8 GMP
-
Schizosaccharomyces pombe
2.4.2.8 GTP
-
Schizosaccharomyces pombe
2.4.2.8 IDP
-
Schizosaccharomyces pombe
2.4.2.8 IMP
-
Schizosaccharomyces pombe
2.4.2.8 ITP weak Schizosaccharomyces pombe
2.4.2.8 Mg2+ inhibitory effects are noncompetitive against 5-phosphoribose 1-diphosphate Schizosaccharomyces pombe
2.4.2.8 additional information mechanism of product inhibition; no inhibition by ADP, ATP, dAMP, UMP, and UTP Schizosaccharomyces pombe
2.4.2.8 UDP weak Schizosaccharomyces pombe
2.4.2.8 XMP
-
Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.7 0.032
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Schizosaccharomyces pombe
2.4.2.7 0.069
-
adenine
-
Schizosaccharomyces pombe
2.4.2.8 0.028
-
guanine
-
Schizosaccharomyces pombe
2.4.2.8 0.1
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Schizosaccharomyces pombe

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.7 Mg2+ absolute requirement Schizosaccharomyces pombe
2.4.2.7 Mg2+ maximal activity at 1-2 mM Schizosaccharomyces pombe
2.4.2.8 Mg2+ absolute requirement Schizosaccharomyces pombe
2.4.2.8 Mg2+ maximal activity at: 0.5-1 mM MgCl2 Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.7 44000
-
gel filtration, 2 forms: 44000 Da and 50000 Da, in presence of 5 mM MgCl2 dissociation into a single form of apparent MW 30000 Schizosaccharomyces pombe
2.4.2.7 50000
-
gel filtration, 2 forms: 44000 Da and 50000 Da, in presence of 5 mM MgCl2 dissociation into a single form of apparent MW 30000 Schizosaccharomyces pombe
2.4.2.8 42000
-
gel filtration in presence of MgCl2 Schizosaccharomyces pombe
2.4.2.8 48000
-
gel filtration in absence of MgCl2 Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Schizosaccharomyces pombe
-
-
-
2.4.2.8 Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7
-
Schizosaccharomyces pombe
2.4.2.8
-
Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.7 18
-
-
Schizosaccharomyces pombe
2.4.2.8 5.75
-
-
Schizosaccharomyces pombe

Storage Stability

EC Number Storage Stability Organism
2.4.2.7 -20°C, purified enzyme, 6 mM 5-phospho-alpha-D-ribose 1-diphosphate, 2 mM MgCl2, stable for at least a month Schizosaccharomyces pombe
2.4.2.8 -20°C, purified enzyme, 6 mM 5-phospho-alpha-D-ribose 1-diphosphate, 2 mM MgCl2, stable for at least a month Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Schizosaccharomyces pombe AMP + diphosphate
-
?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Schizosaccharomyces pombe GMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Schizosaccharomyces pombe IMP + diphosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.7 37
-
assay at Schizosaccharomyces pombe

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.7 60
-
10 min, stable in presence of 10 mM AMP Schizosaccharomyces pombe
2.4.2.8 60
-
1.5 mM GMP, 10 min, complete loss of activity Schizosaccharomyces pombe

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.7 7.5
-
two zones of pH-optima at pH 7.5 and pH 8.5 Schizosaccharomyces pombe
2.4.2.7 8.5
-
two zones of pH-optima at pH 7.5 and 8.5 Schizosaccharomyces pombe
2.4.2.8 7.6 8 2 zones of pH-optima: pH 7.6-8.0 and pH 9.2-9.5 Schizosaccharomyces pombe
2.4.2.8 9.2 9.5 2 zones of pH-optima: pH 7.6-8.0 and pH 9.2-9.5 Schizosaccharomyces pombe