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Literature summary extracted from

  • Gallois, R.; Prevot, J.C.; Clement, A.; Jacob, J.L.
    Purification and characterization of an adenine phosphoribosyltransferase from rubber tree latex. Physiological implications (1996), Plant Physiol. Biochem., 34, 527-537.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.7 additional information no stimulation by ethylene Hevea brasiliensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.7 Ca2+ no inhibition Hevea brasiliensis
2.4.2.7 Cu2+ strong Hevea brasiliensis
2.4.2.7 diphosphate no inhibition Hevea brasiliensis
2.4.2.7 additional information no effect of a variety of sugars, amino acids, organic acids and nucleotides tested, except for AMP, have any effect on the enzyme activity; not affected by PO43- Hevea brasiliensis
2.4.2.7 N-ethylmaleimide strong, DTT or glutathione protect Hevea brasiliensis
2.4.2.7 Na+ no inhibition Hevea brasiliensis
2.4.2.7 p-chloromercuribenzoate complete inhibition at 1 mM, DTT or glutathione protect Hevea brasiliensis
2.4.2.7 SO42- no inhibition Hevea brasiliensis
2.4.2.7 Zn2+ strong Hevea brasiliensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.7 0.0038
-
 adenine
-
 Hevea brasiliensis
2.4.2.7 0.025
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Hevea brasiliensis
2.4.2.7 0.2
-
 Mn2+
-
 Hevea brasiliensis
2.4.2.7 0.3
-
 Mg2+
-
 Hevea brasiliensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.7 cytosol
-
 Hevea brasiliensis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.7 Mg2+ required Hevea brasiliensis
2.4.2.7 Mg2+ Mg2+ most effective Hevea brasiliensis
2.4.2.7 Mg2+ enzyme requires Mn2+ or Mg2+ Hevea brasiliensis
2.4.2.7 Mn2+ required Hevea brasiliensis
2.4.2.7 Mn2+ best activating divalent cation Hevea brasiliensis
2.4.2.7 Mn2+ activity depends on presence of divalent cations, Mn2+ or Mg2+ activates Hevea brasiliensis
2.4.2.7 additional information no effect of K+, Na+, Fe3+, Ca2+ Hevea brasiliensis
2.4.2.7 additional information activity absolutely depends on presence of divalent cations Hevea brasiliensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.7 28000
-
 2 * 28000, SDS-PAGE Hevea brasiliensis
2.4.2.7 54000
-
 native PAGE Hevea brasiliensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Hevea brasiliensis
-
 AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Hevea brasiliensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7
-
 Hevea brasiliensis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.7 AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate mechanism Hevea brasiliensis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.7 latex
-
 Hevea brasiliensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.7 2.2
-
 purified enzyme Hevea brasiliensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Hevea brasiliensis AMP + diphosphate
-
 ?
2.4.2.7 additional information no activity with hypoxanthine, guanine, cytosine Hevea brasiliensis ?
-
 ?
2.4.2.7 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Hevea brasiliensis xanthosine 5'-phosphate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.7 dimer 2 * 28000, SDS-PAGE Hevea brasiliensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.7 50 65
-
 Hevea brasiliensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.7 7
-
-
 Hevea brasiliensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.7 0.002
-
 Cu2+
-
 Hevea brasiliensis
2.4.2.7 0.005
-
 Zn2+
-
 Hevea brasiliensis