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Literature summary extracted from

  • Price, N.P.J.; Kelly, T.M.; Raetz, C.R.H.; Carlson, R.W.
    Biosynthesis of a structurally novel lipid A in Rhizobium leguminosarum: Identification and characterization of six metabolic steps leading from UDP-GlcNAc to 3-deoxy-D-manno-2-octulosonic acid2-lipid IVA (1994), J. Bacteriol., 176, 4646-4655.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.182 Rhizobium leguminosarum
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 biovars phaseoli CE3 and viciae 8401
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.182 additional information
-
 coupled assay, first 3 steps of lipid A biosynthesis Rhizobium leguminosarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.182 UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate 2,3-diacylglucosamine 1-phosphate is lipid X Rhizobium leguminosarum UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
-
 ?
2.4.1.182 UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate lipid X isolated from E. coli Rhizobium leguminosarum UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.182 30
-
 assay at Rhizobium leguminosarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.182 8
-
 assay at Rhizobium leguminosarum