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Literature summary extracted from

  • Lehle, L.; Tanner, W.
    The function of myo-inositol in the biosynthesis of raffinose. Purification and characterization of galactinol:sucrose 6-galactosyltransferase from Vicia faba seeds (1973), Eur. J. Biochem., 38, 103-110.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.82 dithioerythritol stabilizes during purification Vicia faba

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.82 Ag+ 1 mM, strong Vicia faba
2.4.1.82 Al3+ 1 mM, strong Vicia faba
2.4.1.82 Hg2+ 1 mM, strong Vicia faba
2.4.1.82 iodoacetamide 49% inhibition at 1 mM Vicia faba
2.4.1.82 Mn2+
-
 Vicia faba
2.4.1.82 N-ethylmaleimide 90% inhibition at 1 mM Vicia faba
2.4.1.82 Zn2+ 1 mM, strong Vicia faba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.82 1
-
 sucrose cosubstrate galactinol Vicia faba
2.4.1.82 2.9
-
 sucrose + raffinose, exchange reaction Vicia faba
2.4.1.82 7
-
 galactinol + sucrose Vicia faba
2.4.1.82 10
-
 raffinose + sucrose, exchange reaction Vicia faba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.82 cytoplasm
-
 Vicia faba 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.82 80000
-
 gel filtration Vicia faba
2.4.1.82 100000
-
 glycerol density gradient centrifugation Vicia faba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.82 1-alpha-D-galactosyl-myo-inositol + sucrose Vicia faba first step in biosynthesis of raffinose sugars myo-inositol + raffinose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.82 Vicia faba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.82 partial Vicia faba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.82 seed
-
 Vicia faba
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.82 0.0012
-
 crude seed extract Vicia faba
2.4.1.82 0.497
-
 purified enzyme Vicia faba

Storage Stability

EC Number Storage Stability Organism
2.4.1.82 4°C, in crude extract, t1/2: 3 days Vicia faba
2.4.1.82 frozen, purified enzyme, at least 1 month Vicia faba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.82 1-alpha-D-galactosyl-myo-inositol + H2O hydrolysis, in the absence of acceptor substrate Vicia faba myo-inositol + D-galactose
-
 ?
2.4.1.82 1-alpha-D-galactosyl-myo-inositol + sucrose
-
 Vicia faba myo-inositol + raffinose
-
 r
2.4.1.82 1-alpha-D-galactosyl-myo-inositol + sucrose first step in biosynthesis of raffinose sugars Vicia faba myo-inositol + raffinose
-
 ?
2.4.1.82 4-nitrophenol-alpha-D-galactopyranoside + sucrose
-
 Vicia faba 4-nitrophenol + raffinose
-
 ?
2.4.1.82 additional information UDP-galactose is no substrate Vicia faba ?
-
 ?
2.4.1.82 raffinose + sucrose exchange reaction Vicia faba sucrose + raffinose
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.82 galactinol:sucrose 6-galactosyl transferase
-
 Vicia faba

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.82 42
-
-
 Vicia faba

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.82 28 50 about half-maximal activity at 28°C and 50°C Vicia faba

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.82 50
-
 10 min, 80% loss of activity, galactinol and raffinose protect, not sucrose Vicia faba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.82 7
-
-
 Vicia faba

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.82 5.7 7.8 about half-maximal activity at pH 5.7 and pH 7.8 Vicia faba