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Literature summary extracted from

  • Rasmussen, U.B.; Mygind, B.; Nygaard, P.
    Purification and some properties of uracil phosphoribosyltransferase from Escherichia coli K12 (1986), Biochim. Biophys. Acta, 881, 268-275.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.9 GTP effect is pH-dependent, pH 7.5: lowers Km value for 5-phospho-alpha-D-ribose 1-diphosphate and increases Vmax 2fold, no effect on Km for uracil, pH 8.0: no effect Escherichia coli

General Stability

EC Number General Stability Organism
2.4.2.9 2-mercaptoethanol, not essential for stability in long-term storage Escherichia coli
2.4.2.9 ethylene glycol, significantly increases stability with virtually no loss of activity after storage at 4°C for 13 days or after 1 year at -70°C Escherichia coli
2.4.2.9 GTP, 5 mM with 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 2 mM 2-mercaptoethanol, labilizes with only 15% activity remaining after 13 days, labilizing effect abolished in presence of 5-phospho-alpha-D-ribose 1-diphosphate Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.9 additional information no inhibition by diphosphate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.9 0.007
-
 Uracil
-
 Escherichia coli
2.4.2.9 0.3
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.9 23500
-
 3 * 23500, SDS-PAGE Escherichia coli
2.4.2.9 75000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate Escherichia coli pyrimidine salvage enzyme, no de novo synthesis of pyrmidines UMP + diphosphate
-
 ?
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate Escherichia coli SO 1344 pyrimidine salvage enzyme, no de novo synthesis of pyrmidines UMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Escherichia coli
-
 K12
-
2.4.2.9 Escherichia coli
-
 gene upp
-
2.4.2.9 Escherichia coli
-
 and strain SO 1346
-
2.4.2.9 Escherichia coli
-
 enzyme expression is inducible upon uracil starvation
-
2.4.2.9 Escherichia coli SO 1344
-
 and strain SO 1346
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.9 from strain SO 1346 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.9 0.192
-
 strain SO 1346 Escherichia coli
2.4.2.9 6.602
-
 purified enzyme, strain SO 1346 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.4.2.9 4°C for 13 days or 1 year at -70°C, ethylene glycol, significantly increases stability with virtually no loss of activity Escherichia coli
2.4.2.9 4°C, 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 2 mM 2-mercaptoethanol, 44% loss of activity after 8 d Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate 216% of the activity with uracil Escherichia coli 5-fluoro-UMP + diphosphate
-
 r
2.4.2.9 6-azauracil + 5-phospho-alpha-D-ribose 1-diphosphate 97% of the activity with uracil Escherichia coli 6-aza-UMP + diphosphate
-
 ?
2.4.2.9 6-azauracil + 5-phospho-alpha-D-ribose 1-diphosphate 97% of the activity with uracil Escherichia coli SO 1344 6-aza-UMP + diphosphate
-
 ?
2.4.2.9 additional information no substrates: thymine, hypoxanthine Escherichia coli ?
-
 ?
2.4.2.9 additional information no substrates: cytosine, orotic acid Escherichia coli ?
-
 ?
2.4.2.9 additional information no substrates: thymine, hypoxanthine Escherichia coli SO 1344 ?
-
 ?
2.4.2.9 additional information no substrates: cytosine, orotic acid Escherichia coli SO 1344 ?
-
 ?
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate highly specific for uracil and analogues Escherichia coli UMP + diphosphate
-
 r
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate pyrimidine salvage enzyme, no de novo synthesis of pyrmidines Escherichia coli UMP + diphosphate
-
 ?
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate highly specific for uracil and analogues Escherichia coli SO 1344 UMP + diphosphate
-
 r
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate pyrimidine salvage enzyme, no de novo synthesis of pyrmidines Escherichia coli SO 1344 UMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.9 trimer 3 * 23500, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.9 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.9 7.5 8.5
-
 Escherichia coli