Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jensen, H.K.; Mikkelsen, N.; Neuhard, J.
    Recombinant uracil phosphoribosyltransferase from the thermophile Bacillus caldolyticus: expression, purification, and partial characterization (1997), Protein Expr. Purif., 10, 356-364.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 DNA and amino acid sequence determination and analysis, overexpression in enzyme-deficient Escherichia coli [Bacillus] caldolyticus

General Stability

EC Number General Stability Organism
2.4.2.9 bovine serum albumin at 2 mg/ml stabilizes [Bacillus] caldolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.9 0.002
-
 Uracil
-
 [Bacillus] caldolyticus
2.4.2.9 0.05
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 [Bacillus] caldolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.9 46000
-
 gel filtration [Bacillus] caldolyticus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Bacillus subtilis
-
-
-
2.4.2.9 Bacillus subtilis 168
-
-
-
2.4.2.9 [Bacillus] caldolyticus P70881 gene upp
-
2.4.2.9 [Bacillus] caldolyticus P70881 strain DSM 405
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.9 recombinant from enzyme-deficient Escherichia coli [Bacillus] caldolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.9 0.07
-
 37°C Bacillus subtilis
2.4.2.9 0.09
-
 60°C [Bacillus] caldolyticus
2.4.2.9 19.2
-
 purified recombinant enzyme, 37°C [Bacillus] caldolyticus

Storage Stability

EC Number Storage Stability Organism
2.4.2.9 0°C, enzyme concentration 0.001 mg/ml, 50% loss of activity in 1 h [Bacillus] caldolyticus
2.4.2.9 4°C, concentrated enzyme is stable for several months, on dilution occurs rapid loss of activity [Bacillus] caldolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Bacillus subtilis UMP + diphosphate
-
 ?
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 [Bacillus] caldolyticus UMP + diphosphate
-
 ?
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Bacillus subtilis 168 UMP + diphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.9 60
-
 recombinant enzyme [Bacillus] caldolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.9 45
-
 10 min, 90% remaining activity Bacillus subtilis
2.4.2.9 55
-
 10 min, 20% remaining activity Bacillus subtilis
2.4.2.9 60
-
 10 min, inactivation Bacillus subtilis
2.4.2.9 70
-
 10 min, 70% remaining activity [Bacillus] caldolyticus
2.4.2.9 75
-
 10 min, 5% remaining activity [Bacillus] caldolyticus
2.4.2.9 80
-
 10 min, inactivation [Bacillus] caldolyticus