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Literature summary extracted from

  • Veres, G.; Monostori, E.; Rasko, I.
    Purification and characterisation of chicken brain hypoxanthine-guanine phosphoribosyltransferase (1985), FEBS Lett., 184, 299-303.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.0018
-
guanine
-
Gallus gallus
2.4.2.8 0.0052
-
hypoxanthine
-
Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.8 Mg2+ absolute requirement for Mg2+ or Mn2+ Gallus gallus
2.4.2.8 Mn2+ absolute requirement for Mg2+ or Mn2+ Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.8 26000
-
4 * 26000, SDS-PAGE Gallus gallus
2.4.2.8 85000
-
gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8 2 methods Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.8 brain
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.8 698.1
-
purified enzyme, method 1 Gallus gallus
2.4.2.8 705
-
purified enzyme, immunopurification Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Gallus gallus GMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Gallus gallus IMP + diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.2.8 tetramer 4 * 26000, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.8 37
-
assay at Gallus gallus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.8 85
-
half-life: 3 min Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.8 10
-
-
Gallus gallus