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Literature summary extracted from

  • Heroux, A.; White, E.L.; Ross, L.J.; Davis, R.L.; Borhani, D.W.
    Crystal structure of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase with XMP, pyrophosphate, and two Mg2+ Ions Bound: insights into the catalytic mechanism (1999), Biochemistry, 38, 14495-14506.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.8 mutant enzyme D150A, crystallization complexed with xanthosine 5'-monophosphate, diphosphate and 2 Mg2+, post transition state structure analysis, active site structure Toxoplasma gondii

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.8 D150A reduced activity compared to wild-type, kcat for hypoxanthine, guanine, and xanthine are reduced by 11fold, 296fold, and 8.6fold, respectively, Km value for alpha-D-5-phosphoribosyl 1-diphosphate is reduced by 6.5fold Toxoplasma gondii

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Toxoplasma gondii Q26997
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.8 IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate catalytic mechanism Toxoplasma gondii
a
2.4.2.8 IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate active site structure Toxoplasma gondii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Toxoplasma gondii GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Toxoplasma gondii IMP + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Toxoplasma gondii XMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.8 More structure determination and analysis Toxoplasma gondii
2.4.2.8 tetramer
-
 Toxoplasma gondii