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Literature summary extracted from

  • Xu, Y.; Eads, J.; Sacchettini, J.C.; Grubmeyer, C.
    Kinetic mechanism of human hypoxanthine-guanine phosphoribosyltransferase: rapid phosphoribosyl transfer chemistry (1997), Biochemistry, 36, 3700-3712.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.8 medicine potential target for antiparasitic chemotherapy Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.8
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 additional information
-
 additional information kinetics Homo sapiens
2.4.2.8 0.0024
-
 hypoxanthine recombinant enzyme Homo sapiens
2.4.2.8 0.0035
-
 guanine recombinant enzyme Homo sapiens
2.4.2.8 0.0054
-
 inosine monophosphate recombinant enzyme Homo sapiens
2.4.2.8 0.025
-
 diphosphate recombinant enzyme, with inosine monophosphate Homo sapiens
2.4.2.8 0.035
-
 5-phospho-alpha-D-ribose 1-diphosphate recombinant enzyme, with hypoxanthine Homo sapiens
2.4.2.8 0.065
-
 5-phospho-alpha-D-ribose 1-diphosphate recombinant enzyme, with guanine Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.8 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate Homo sapiens
-
 GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Homo sapiens
-
 IMP + diphosphate
-
 ?
2.4.2.8 additional information Homo sapiens locus of Lesch-Nyhan syndrome, activator of the prodrugs 6-mercaptopurine and allopurinol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8 recombinant enzyme Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.8 IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate kinetic mechanism Homo sapiens
a
2.4.2.8 IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate catalytic mechanism Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.8 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.8 additional information
-
 kinetics Homo sapiens
2.4.2.8 0.57 0.76 recombinant enzyme, reverse reaction Homo sapiens
2.4.2.8 21 23 recombinant enzyme, forward reaction Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens GMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate guanine is utilized more rapidly than hypoxanthine Homo sapiens GMP + diphosphate
-
 r
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate equilibrium lies far in direction of IMP formation Homo sapiens IMP + diphosphate
-
 r
2.4.2.8 additional information kinetic study Homo sapiens ?
-
 ?
2.4.2.8 additional information locus of Lesch-Nyhan syndrome, activator of the prodrugs 6-mercaptopurine and allopurinol Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.8 tetramer
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.8 30
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.8 additional information
-
 additional information kinetics Homo sapiens
2.4.2.8 0.23 0.3 inosine monophosphate recombinant enzyme, reverse reaction Homo sapiens
2.4.2.8 8.5 9.3 hypoxanthine recombinant enzyme Homo sapiens