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Literature summary extracted from

  • Avraham, Y.; Yashphe, J.; Grossowicz, N.
    Thymidine phosphorylase and uridine phosphorylase of Lactobacillus casei (1988), FEMS Microbiol. Lett., 56, 29-34.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.3 3-O-methyl-alpha-D-glucopyranose
-
 Lacticaseibacillus casei
2.4.2.3 5-fluoro-2'-deoxyuridine
-
 Lacticaseibacillus casei
2.4.2.3 6-Methyluracil
-
 Lacticaseibacillus casei
2.4.2.3 alpha-D-ribose 1-phosphate
-
 Lacticaseibacillus casei
2.4.2.3 thymidine 5-monophosphate
-
 Lacticaseibacillus casei
2.4.2.3 Uracil
-
 Lacticaseibacillus casei
2.4.2.4 5-bromo-2-deoxyuridine 100% inhibition at 10 mM Lacticaseibacillus casei
2.4.2.4 5-fluorodeoxyuridine 80% inhibition at 10 mM Lacticaseibacillus casei
2.4.2.4 6-Methyluracil competitive, 86% inhibition at 10 mM and 36% inhibition at 1 mM Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.3 3.8
-
 uridine
-
 Lacticaseibacillus casei
2.4.2.4 0.00166
-
 thymidine
-
 Lacticaseibacillus casei
2.4.2.4 0.1
-
 phosphate
-
 Lacticaseibacillus casei

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Lacticaseibacillus casei
-
-
-
2.4.2.4 Lacticaseibacillus casei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3
-
 Lacticaseibacillus casei
2.4.2.4
-
 Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.3 4278
-
-
 Lacticaseibacillus casei
2.4.2.4 111
-
-
 Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 uridine + phosphate
-
 Lacticaseibacillus casei uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.4 thymidine + phosphate
-
 Lacticaseibacillus casei thymine + 2-deoxy-D-ribose 1-phosphate
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.3 55
-
 40 min, 95% loss of activity Lacticaseibacillus casei
2.4.2.3 60
-
 20 min, complete loss of activity Lacticaseibacillus casei
2.4.2.4 55
-
 40 min, 10% loss of activity Lacticaseibacillus casei
2.4.2.4 60
-
 20 min, complete loss of activity Lacticaseibacillus casei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.3 7
-
-
 Lacticaseibacillus casei
2.4.2.4 6
-
-
 Lacticaseibacillus casei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.3 1.36
-
 3-O-methyl-alpha-D-glucopyranose
-
 Lacticaseibacillus casei
2.4.2.3 1.45
-
 Uracil
-
 Lacticaseibacillus casei
2.4.2.3 3.5
-
 alpha-D-ribose 1-phosphate
-
 Lacticaseibacillus casei
2.4.2.4 1.6
-
 6-Methyluracil
-
 Lacticaseibacillus casei