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Literature summary extracted from

  • Ivens, A.; Mayans, O.; Szadkowski, H.; Wilmanns, M.; Kirschner, K.
    Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus (2001), Eur. J. Biochem., 268, 2246-2252.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.18
-
 Saccharolobus solfataricus
2.4.2.18 expression in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.18 hanging drop vapor diffusion method Saccharolobus solfataricus
2.4.2.18 hanging-drop method, crystals of ssTrpD diffract to better than 2.6 A resolution Saccharolobus solfataricus

General Stability

EC Number General Stability Organism
2.4.2.18 the enzyme is either digested by trypsin, V8-protease or thermolysin after incubation for 1 h at 25°C but not by thermolysin at 70°C Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.18 0.000005
-
 anthranilate at 87°C, Km decreases at lower temperatures Saccharolobus solfataricus
2.4.2.18 0.002
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C Saccharolobus solfataricus
2.4.2.18 0.002
-
 anthranilate 25°C Saccharolobus solfataricus
2.4.2.18 0.0026
-
 5-phospho-alpha-D-ribose 1-diphosphate 40°C Saccharolobus solfataricus
2.4.2.18 0.0026
-
 anthranilate 40°C Saccharolobus solfataricus
2.4.2.18 0.0032
-
 5-phospho-alpha-D-ribose 1-diphosphate 50°C Saccharolobus solfataricus
2.4.2.18 0.0032
-
 anthranilate 50°C Saccharolobus solfataricus
2.4.2.18 0.0035
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C Saccharolobus solfataricus
2.4.2.18 0.0035
-
 anthranilate 60°C Saccharolobus solfataricus
2.4.2.18 0.005
-
 5-phospho-alpha-D-ribose 1-diphosphate 87°C Saccharolobus solfataricus
2.4.2.18 0.005
-
 anthranilate 87°C Saccharolobus solfataricus
2.4.2.18 0.01
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.18 73300
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate Saccharolobus solfataricus enzyme of tryptophan biosynthesis N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.18 Saccharolobus solfataricus
-
-
-
2.4.2.18 Saccharolobus solfataricus P50384
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.18
-
 Saccharolobus solfataricus
2.4.2.18 95% pure Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus solfataricus N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate enzyme of tryptophan biosynthesis Saccharolobus solfataricus N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.18 dimer
-
 Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.18 ssTrpD
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.18 70
-
 50% loss of activity after 69 min Saccharolobus solfataricus
2.4.2.18 70
-
 t1/2: 69 min, addition of anthranilate has no stabilizing effect Saccharolobus solfataricus
2.4.2.18 85
-
 50% loss of activity after 35 min Saccharolobus solfataricus
2.4.2.18 85
-
 t1/2: 35 min, addition of anthranilate has no stabilizing effect Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.18 0.014
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C Saccharolobus solfataricus
2.4.2.18 0.014
-
 anthranilate 25°C Saccharolobus solfataricus
2.4.2.18 0.014
-
 anthranilate at 25°C Saccharolobus solfataricus
2.4.2.18 0.035
-
 anthranilate at 40°C Saccharolobus solfataricus
2.4.2.18 0.035
-
 5-phospho-alpha-D-ribose 1-diphosphate 40°C Saccharolobus solfataricus
2.4.2.18 0.035
-
 anthranilate 40°C Saccharolobus solfataricus
2.4.2.18 0.067
-
 anthranilate at 50°C Saccharolobus solfataricus
2.4.2.18 0.067
-
 5-phospho-alpha-D-ribose 1-diphosphate 50°C Saccharolobus solfataricus
2.4.2.18 0.067
-
 anthranilate 50°C Saccharolobus solfataricus
2.4.2.18 0.105
-
 5-phospho-alpha-D-ribose 1-diphosphate 60°C Saccharolobus solfataricus
2.4.2.18 0.105
-
 anthranilate 60°C Saccharolobus solfataricus
2.4.2.18 0.105
-
 anthranilate at 60°C Saccharolobus solfataricus
2.4.2.18 0.4
-
 anthranilate at 87°C Saccharolobus solfataricus
2.4.2.18 0.4
-
 5-phospho-alpha-D-ribose 1-diphosphate 87°C Saccharolobus solfataricus
2.4.2.18 0.4
-
 anthranilate 87°C Saccharolobus solfataricus