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Literature summary extracted from

  • Light, D.R.; Dennis, M.S.
    Purification of a prenyltransferase that elongates cis-polyisoprene rubber from the latex of Hevea brasiliensis (1989), J. Biol. Chem., 264, 18589-18597.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.20 2-mercaptoethanol 10 mM, not as effective as dithiothreitol Hevea brasiliensis
2.5.1.20 dithiothreitol 10 mM, required for maximal stability Hevea brasiliensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.20 Co2+ 0.1-1 mM, complete inhibition Hevea brasiliensis
2.5.1.20 diphosphate
-
Hevea brasiliensis
2.5.1.20 iodoacetamide complete inhibition Hevea brasiliensis
2.5.1.20 iodoacetic acid
-
Hevea brasiliensis
2.5.1.20 Sodium diphosphate
-
Hevea brasiliensis
2.5.1.20 Sodium phosphate
-
Hevea brasiliensis
2.5.1.20 Thiol alkylating agents
-
Hevea brasiliensis
2.5.1.20 Zn2+ 0.1-1 mM, complete inhibition Hevea brasiliensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.20 Ca2+ maximal stimulation at 5 mM Hevea brasiliensis
2.5.1.20 Mg2+ required for activity Hevea brasiliensis
2.5.1.20 Mg2+ maximal stimulation at 1 mM Hevea brasiliensis
2.5.1.20 Mn2+ stimulates, maximal activity at 1 mM Hevea brasiliensis
2.5.1.20 Pb2+ maximal stimulation at 5 mM Hevea brasiliensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.20 38000
-
2 * 38000, SDS-PAGE Hevea brasiliensis
2.5.1.20 70000
-
gel filtration Hevea brasiliensis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.20 Hevea brasiliensis
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.5.1.20 thiols stabilize Hevea brasiliensis

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.20 ammonium sulfate, DE52, Sephacryl S-200, hexyl-Sepharose, Mono Q Hevea brasiliensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.20 latex
-
Hevea brasiliensis
-
2.5.1.20 leaf
-
Hevea brasiliensis
-

Storage Stability

EC Number Storage Stability Organism
2.5.1.20 -80°C, 25 mM Tris-HCl, pH 8, 10 mM dithiothreitol, 1 mM MgSO4, 30% glycerol, 0.1% sodium azide, at least 6 months, no loss of activity Hevea brasiliensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.20 dimethylallyl diphosphate + isopentenyl diphosphate
-
Hevea brasiliensis (E,E)-farnesyl diphosphate + (E)-geranyl diphosphate in the absence of rubber ?
2.5.1.20 isopentenyl diphosphate + cis-1,4-polyisoprene of rubber particles Hevea brasiliensis diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.20 dimer 2 * 38000, SDS-PAGE Hevea brasiliensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.20 25
-
assay at Hevea brasiliensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.20 5
-
Sodium diphosphate
-
Hevea brasiliensis
2.5.1.20 200
-
diphosphate
-
Hevea brasiliensis