EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.29 | 0.009 | - |
isopentenyl diphosphate | - |
Phycomyces blakesleeanus | |
2.5.1.29 | 0.06 | - |
farnesyl diphosphate | - |
Phycomyces blakesleeanus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.29 | 30000 | - |
2 * 30000, the native enzyme consists of 2 identical subunits with a tendency to dissociate, SDS-PAGE | Phycomyces blakesleeanus |
2.5.1.29 | 60000 | - |
gel filtration, enzyme consists of 2 identical subunits of 30000 Da with a tendency to dissociate | Phycomyces blakesleeanus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.29 | (E,E)-farnesyl diphosphate + isopentenyl diphosphate | Phycomyces blakesleeanus | preferred allylic substrate | geranylgeranyl diphosphate + diphosphate | - |
? | |
2.5.1.29 | dimethylallyl diphosphate + 3 isopentenyl diphosphate | Phycomyces blakesleeanus | less than 5% of the activity with farnesyl diphosphate | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
2.5.1.29 | geranyl diphosphate + 2 isopentenyl diphosphate | Phycomyces blakesleeanus | less than 5% of the activity with farnesyl diphosphate | geranylgeranyl diphosphate + 2 diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.29 | Phycomyces blakesleeanus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.29 | polyethylene glycol, DE-52 cellulose, DEAE Trisacryl-M, Reactive blue 4-agarose, Reactive red 120-agarose | Phycomyces blakesleeanus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.29 | 0.3 | - |
- |
Phycomyces blakesleeanus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.29 | (E,E)-farnesyl diphosphate + isopentenyl diphosphate | preferred allylic substrate | Phycomyces blakesleeanus | geranylgeranyl diphosphate + diphosphate | - |
? | |
2.5.1.29 | dimethylallyl diphosphate + 3 isopentenyl diphosphate | less than 5% of the activity with farnesyl diphosphate | Phycomyces blakesleeanus | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
2.5.1.29 | geranyl diphosphate + 2 isopentenyl diphosphate | less than 5% of the activity with farnesyl diphosphate | Phycomyces blakesleeanus | geranylgeranyl diphosphate + 2 diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.29 | dimer | 2 * 30000, the native enzyme consists of 2 identical subunits with a tendency to dissociate, SDS-PAGE | Phycomyces blakesleeanus |