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Literature summary extracted from

  • Kawasaki, T.; Hamano, Y.; Kuzuyama, T.; Itoh, N.; Seto, H.; Dairi, T.
    Interconversion of the product specificity of type I eubacterial farnesyl diphosphate synthase and geranylgeranyl diphosphate synthase through one amino acid substitution (2003), J. Biochem., 133, 83-91.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.10
-
 Streptomyces argenteolus
2.5.1.29 overexpression in Escherichia coli Kitasatospora griseola

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.10 S50L mutant enzyme produces prenyl diphosphates with the same chain lengths as the wild-type enzyme does. 1.3-1.4fold decrease in ratio of Vmax to Km-value for dimethylallyl diphosphate and isopentenyl diphosphate Streptomyces argenteolus
2.5.1.29 A57L mutated enzyme produces mainly farnesyl diphosphate Kitasatospora griseola
2.5.1.29 L50S similar activity as wild-type Kitasatospora griseola
2.5.1.29 S4F very weak activity Kitasatospora griseola
2.5.1.29 V8A similar activity as wild-type Kitasatospora griseola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.10 0.0159
-
 dimethylallyl diphosphate mutant enzyme L58A Streptomyces argenteolus
2.5.1.10 0.0531
-
 isopentenyl diphosphate cosubstrate: dimethylallyl diphosphate, wild-type enzyme Streptomyces argenteolus
2.5.1.10 0.0666
-
 dimethylallyl diphosphate wild-type enzyme Streptomyces argenteolus
2.5.1.10 0.0705
-
 farnesyl diphosphate mutant enzyme L58A Streptomyces argenteolus
2.5.1.10 0.221
-
 isopentenyl diphosphate cosubstrate: farnesyl diphosphate, mutant enzyme L58A Streptomyces argenteolus
2.5.1.29 0.036
-
 isopentenyl diphosphate cosubstrate farnesyl diphosphate Kitasatospora griseola
2.5.1.29 0.066
-
 dimethylallyl diphosphate
-
 Kitasatospora griseola
2.5.1.29 0.099
-
 dimethylallyl diphosphate A57L mutant enzyme Kitasatospora griseola
2.5.1.29 0.108
-
 isopentenyl diphosphate cosubstrate dimethylallyl diphosphate Kitasatospora griseola
2.5.1.29 0.121
-
 farnesyl diphosphate
-
 Kitasatospora griseola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate Kitasatospora griseola
-
 geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate Kitasatospora griseola MF730-N6
-
 geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate Kitasatospora griseola
-
 geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate Kitasatospora griseola MF730-N6
-
 geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate Kitasatospora griseola
-
 geranylgeranyl diphosphate + 2 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate Kitasatospora griseola MF730-N6
-
 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Streptomyces argenteolus
-
-
-
2.5.1.29 Kitasatospora griseola
-
 MF730-N6
-
2.5.1.29 Kitasatospora griseola MF730-N6
-
 MF730-N6
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 dimethylallyl diphosphate + 2 isopentenyl diphosphate
-
 Streptomyces argenteolus 2 diphosphate + trans,trans-farnesyl diphosphate
-
 ?
2.5.1.10 dimethylallyl diphosphate + isopentenyl diphosphate
-
 Streptomyces argenteolus diphosphate + geranyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
 Streptomyces argenteolus diphosphate + trans,trans-farnesyl diphosphate
-
 ?
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Kitasatospora griseola geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Kitasatospora griseola MF730-N6 geranylgeranyl diphosphate + diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Kitasatospora griseola geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Kitasatospora griseola MF730-N6 geranylgeranyl diphosphate + 3 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
 Kitasatospora griseola geranylgeranyl diphosphate + 2 diphosphate
-
 ?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
 Kitasatospora griseola MF730-N6 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.10 farnesyl diphosphate synthase
-
 Streptomyces argenteolus
2.5.1.10 FDPSase
-
 Streptomyces argenteolus
2.5.1.10 type I farnesyl diphosphate synthase
-
 Streptomyces argenteolus