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Literature summary extracted from

  • Schwartz, W.N.; Barrett, A.J.
    Human cathepsin H (1980), Biochem. J., 191, 487-497.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.16 4-aminophenylmercuric acetate 93% inhibition at 1 mM Homo sapiens
3.4.22.16 Benzyloxycarbonyl-Phe-Phe-CHN2 31% inhibition at 0.01 mM Homo sapiens
3.4.22.16 chymostatin B 47% inhibition at 0.016 mM Homo sapiens
3.4.22.16 Hyamine 1622 52% inhibition at 1 mM Homo sapiens
3.4.22.16 iodoacetamide 99% inhibition at 1 mM Homo sapiens
3.4.22.16 iodoacetic acid 99% inhibition at 1 mM Homo sapiens
3.4.22.16 iodoacetic acid
-
 Rattus norvegicus
3.4.22.16 Leu-CH2Cl 85% inhibition at 0.001 mM Homo sapiens
3.4.22.16 leupeptin 29% inhibition at 0.021 mM Homo sapiens
3.4.22.16 leupeptin
-
 Rattus norvegicus
3.4.22.16 N-ethylmaleimide 69% inhibition at 1 mM Homo sapiens
3.4.22.16 puromycin 36% inhibition at 1 mM Homo sapiens
3.4.22.16 Soybean trypsin inhibitor non-specific Homo sapiens
3.4.22.16 tosyl-Leu-CH2Cl 60% inhibition at 0.01 mM Homo sapiens
3.4.22.16 tosyl-Leu-CH2Cl
-
 Rattus norvegicus
3.4.22.16 tosyl-Phe-CH2Cl 79% inhibition at 0.01 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.16 0.097
-
 Arg-2-naphthylamide
-
 Homo sapiens
3.4.22.16 0.15
-
 Arg-4-methylcoumaryl-7-amide
-
 Homo sapiens
3.4.22.16 0.637
-
 benzoyl-Arg-2-naphthylamide
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.16 28000
-
-
 Homo sapiens
3.4.22.16 28000
-
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.16 Homo sapiens
-
-
-
3.4.22.16 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.16 side-chain modification
-
 Homo sapiens
3.4.22.16 side-chain modification
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.16
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.16 liver
-
 Homo sapiens
-
3.4.22.16 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.16 additional information
-
-
 Homo sapiens
3.4.22.16 additional information
-
-
 Rattus norvegicus
3.4.22.16 0.15
-
 substrate benzyloxycarbonyl-Arg-Arg-2-naphthylamide Homo sapiens
3.4.22.16 1.73
-
 substrate benzoyl-DL-Arg-2-naphthylamide Homo sapiens
3.4.22.16 1.95
-
 substrate Leu-2-naphthylamide Homo sapiens
3.4.22.16 3.5
-
 substrate Arg-2-naphthylamide Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.4.22.16 4°C or -20°C, 50-100 mM Na-acetate buffer, pH 5.5, 1 mM Na2-EDTA, 0.1% Brij 35, 5 mM Na-tetrathionate, 1 month, 0-10% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.16 alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O
-
 Homo sapiens alpha-N-benzoyl-DL-arginine + beta-naphthylamine
-
 ?
3.4.22.16 Arg-2-naphthylamide + H2O
-
 Homo sapiens Arg + 2-naphthylamine
-
 ir
3.4.22.16 Arg-2-naphthylamide + H2O
-
 Rattus norvegicus Arg + 2-naphthylamine
-
 ir
3.4.22.16 Arg-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.16 benzoyl-DL-Arg-2-naphthylamide + H2O
-
 Homo sapiens benzoyl-DL-Arg + 2-naphthylamine
-
 ir
3.4.22.16 benzoyl-DL-Arg-2-naphthylamide + H2O
-
 Rattus norvegicus benzoyl-DL-Arg + 2-naphthylamine
-
 ir
3.4.22.16 Leu-2-naphthylamide + H2O
-
 Homo sapiens Leu + 2-naphthylamine
-
 ir
3.4.22.16 Leu-2-naphthylamide + H2O
-
 Rattus norvegicus Leu + 2-naphthylamine
-
 ir

Subunits

EC Number Subunits Comment Organism
3.4.22.16 monomer
-
 Homo sapiens
3.4.22.16 monomer
-
 Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.16 alpha-N-benzoyl-arginine 2-naphthylamide hydrolase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.16 40
-
-
 Homo sapiens
3.4.22.16 40
-
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.16 50
-
 30 min, 15% loss of activity Homo sapiens
3.4.22.16 60
-
 30 min, 80% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.16 5 6 substrate azocasein Homo sapiens
3.4.22.16 5 6 substrate azocasein Rattus norvegicus
3.4.22.16 6.8
-
-
 Rattus norvegicus
3.4.22.16 6.8
-
 synthetic substrates Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.16 5 8 less than 20% of maximal activity above and below Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.16 additional information
-
 irreversibly inactivated at alkaline pH Homo sapiens
3.4.22.16 7
-
 irreversible inactivation above Homo sapiens