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Literature summary extracted from

  • Pike, R.; McGraw, W.; Potempa, J.; Travis, J.
    Lysine- and arginine-specific proteinases from Porphyromonas gingivalis. Isolation, characterization, and evidence for the existence of complexes with hemagglutinins (1994), J. Biol. Chem., 269, 406-411.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.47 2-mercaptoethanol
-
 Porphyromonas gingivalis
3.4.22.47 cysteine most effective reducing agent for activation, maximal activation at 50 mM Porphyromonas gingivalis
3.4.22.47 dithiothreitol
-
 Porphyromonas gingivalis
3.4.22.47 glutathione
-
 Porphyromonas gingivalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.47 Gly-Gly 200 mM, 50% inhibition Porphyromonas gingivalis
3.4.22.47 iodoacetamide 10 mM, complete inhibition Porphyromonas gingivalis
3.4.22.47 N-alpha-p-tosyl-L-lysine chloromethyl ketone 0.1 mM, complete inhibition Porphyromonas gingivalis
3.4.22.47 N-ethylmaleimide 10 mM, complete inhibition Porphyromonas gingivalis
3.4.22.47 Phe-Pro-Arg-chloromethyl ketone 0.1 mM, 95% inhibition Porphyromonas gingivalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.47 0.05
-
 N-p-tosyl-Gly-Pro-Lys-p-nitroanilide pH 8.5, 37°C Porphyromonas gingivalis
3.4.22.47 0.126
-
 D-Val-Phe-Lys-p-nitroanilide pH 8.5, 37°C Porphyromonas gingivalis
3.4.22.47 0.18
-
 benzyloxycarbonyl-L-Lys-p-nitroanilide pH 8.5, 37°C Porphyromonas gingivalis
3.4.22.47 0.2
-
 D-Val-Leu-Lys-p-nitroanilide pH 8.5, 37°C Porphyromonas gingivalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.47 60000
-
 x * 60000, SDS-PAGE Porphyromonas gingivalis
3.4.22.47 105000
-
 gel filtration without boiling Porphyromonas gingivalis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.47 Porphyromonas gingivalis
-
-
-
3.4.22.47 Porphyromonas gingivalis H66
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.47 acetone precipitate, Sephadex G-150, arginine-Sepharose Porphyromonas gingivalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.47 acid-soluble human placental type I collagen + H2O
-
 Porphyromonas gingivalis fragments of acid-soluble human placental type I collagen
-
 ?
3.4.22.47 adrenocorticotropic hormone fragment 11-24 + H2O
-
 Porphyromonas gingivalis fragments of adrenocorticotropic hormone fragment 11-24
-
 ?
3.4.22.47 benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O
-
 Porphyromonas gingivalis benzyloxycarbonyl-L-Lys + p-nitroaniline + H2O
-
 ?
3.4.22.47 benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O
-
 Porphyromonas gingivalis H66 benzyloxycarbonyl-L-Lys + p-nitroaniline + H2O
-
 ?
3.4.22.47 beta-endorphin + H2O
-
 Porphyromonas gingivalis fragments of beta-endorphin
-
 ?
3.4.22.47 bovine hemoglobin + H2O
-
 Porphyromonas gingivalis fragments of bovine hemoglobin
-
 ?
3.4.22.47 bovine serum albumin + H2O
-
 Porphyromonas gingivalis fragments of bovine serum albumin
-
 ?
3.4.22.47 casein + H2O
-
 Porphyromonas gingivalis fragments of casein
-
 ?
3.4.22.47 D-Val-Leu-Lys-p-nitroanilide + H2O
-
 Porphyromonas gingivalis D-Val-Leu-Lys + p-nitroaniline
-
 ?
3.4.22.47 D-Val-Leu-Lys-p-nitroanilide + H2O
-
 Porphyromonas gingivalis H66 D-Val-Leu-Lys + p-nitroaniline
-
 ?
3.4.22.47 D-Val-Phe-Lys-p-nitroanilide + H2O
-
 Porphyromonas gingivalis D-Val-Phe-Lys + p-nitroaniline
-
 ?
3.4.22.47 D-Val-Phe-Lys-p-nitroanilide + H2O
-
 Porphyromonas gingivalis H66 D-Val-Phe-Lys + p-nitroaniline
-
 ?
3.4.22.47 human IgA + H2O
-
 Porphyromonas gingivalis cleaved human IgA
-
 ?
3.4.22.47 human IgG + H2O
-
 Porphyromonas gingivalis fragments of human IgG
-
 ?
3.4.22.47 melittin + H2O
-
 Porphyromonas gingivalis fragments of melittin
-
 ?
3.4.22.47 Met-Lys-bradykinin + H2O
-
 Porphyromonas gingivalis fragments of Met-Lys-bradykinin
-
 ?
3.4.22.47 N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O gingipain K cleaves specifically on the C-terminal side of peptide substrates Porphyromonas gingivalis N-p-tosyl-Gly-Pro-Lys + p-nitroaniline
-
 ?
3.4.22.47 N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O gingipain K cleaves specifically on the C-terminal side of peptide substrates Porphyromonas gingivalis H66 N-p-tosyl-Gly-Pro-Lys + p-nitroaniline
-
 ?
3.4.22.47 neurotensin + H2O
-
 Porphyromonas gingivalis pGlu-Leu-Tyr-Glu-Asn-Lys + Pro-Arg-Arg-Pro-Tyr-Ile-Leu
-
 ?
3.4.22.47 neurotensin + H2O
-
 Porphyromonas gingivalis H66 pGlu-Leu-Tyr-Glu-Asn-Lys + Pro-Arg-Arg-Pro-Tyr-Ile-Leu
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.47 ? x * 60000, SDS-PAGE Porphyromonas gingivalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.47 25
-
 no loss of activity Porphyromonas gingivalis
3.4.22.47 37
-
 no loss of activity Porphyromonas gingivalis
3.4.22.47 60
-
 rapid inactivation Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.47 8
-
 hydrolysis of small synthetic peptides Porphyromonas gingivalis
3.4.22.47 8.5
-
 with protein substrates like azocasein Porphyromonas gingivalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.47 5 9 stable for several hours in the absence of cysteine, rapid loss of activity below pH 8.0 in the presence of cysteine Porphyromonas gingivalis