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Literature summary extracted from

  • Pfitzner, A.; Stoeckigt, J.
    Characterization of polyneuridine aldehyde esterase, a key enzyme in the biosynthesis of sarpagine/ajmaline-type alkaloids (1983), Planta Med., 48, 221-227.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.78 2,2'-dithiodipyridine
-
 Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 5,5'-dithio-bis-(2-nitrobenzoate)
-
 Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 iodoacetamide 100% inhibition at 5 mM Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 MgCl2 50% inhibition at 0.25 mM Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 MnCl2 50% inhibition at 0.25 mM Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 ZnCl2 50% inhibition at 0.25 mM Rauvolfia serpentina Benth. ex Kurz

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.78 1.25
-
 polyneuridine aldehyde
-
 Rauvolfia serpentina Benth. ex Kurz

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.78 polyneuridine aldehyde + H2O Rauvolfia serpentina Benth. ex Kurz key enzyme in the biosynthesis of sarpagine/ajmaline type alkaloids 16-epivellosimine + CO2 + methanol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.78 Rauvolfia serpentina Benth. ex Kurz
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.78
-
 Rauvolfia serpentina Benth. ex Kurz

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
3.1.1.78 0°C, 5 months, 0.2 M 2-mercaptoethanol, 100% activity Rauvolfia serpentina Benth. ex Kurz

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.78 akuammidine aldehyde + H2O
-
 Rauvolfia serpentina Benth. ex Kurz vellosiminol + CO2 + methanol
-
 ?
3.1.1.78 polyneuridine aldehyde + H2O
-
 Rauvolfia serpentina Benth. ex Kurz 16-epivellosimine + CO2 + methanol
-
 ?
3.1.1.78 polyneuridine aldehyde + H2O key enzyme in the biosynthesis of sarpagine/ajmaline type alkaloids Rauvolfia serpentina Benth. ex Kurz 16-epivellosimine + CO2 + methanol
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.78 6.5
-
-
 Rauvolfia serpentina Benth. ex Kurz

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.78 4 8.5
-
 Rauvolfia serpentina Benth. ex Kurz