EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.276 | overexpression in Escherichia coli JM109 | Escherichia coli |
1.1.1.276 | overexpression in Escherichia coli JM109 | Saccharomyces cerevisiae |
1.1.1.381 | expression in Escherichia coli JM109 | Escherichia coli |
1.1.1.381 | expression in Escherichia coli JM109 | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.276 | additional information | - |
additional information | substrate specificity | Escherichia coli | |
1.1.1.276 | additional information | - |
additional information | substrate specificity | Saccharomyces cerevisiae | |
1.1.1.276 | 0.5 | - |
NADP+ | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.276 | 0.54 | - |
NADP+ | pH 9.0, 30°C | Escherichia coli | |
1.1.1.276 | 3 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.276 | 29 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.276 | 40 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.276 | 95 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 0.54 | - |
NADP+ | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 3 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 7 | - |
D-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 29 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 33 | - |
L-Glycerate | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 36 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 40 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 50 | - |
D-glycerate | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 57 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 60 | - |
D-threonine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 60 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 61 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 69 | - |
D-serine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 71 | - |
D-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 95 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 95 | - |
L-3-hydroxybutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.276 | 27000 | - |
4 * 27000, recombinant enzyme, SDS-PAGE | Escherichia coli |
1.1.1.276 | 29000 | - |
4 * 29000, recombinant enzyme, SDS-PAGE | Saccharomyces cerevisiae |
1.1.1.276 | 105000 | - |
recombinant enzyme, gel filtration | Escherichia coli |
1.1.1.276 | 110000 | - |
recombinant enzyme, gel filtration | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.276 | L-serine + NADP+ | Escherichia coli | - |
2-aminomalonate semialdehyde + NADPH | - |
? | |
1.1.1.276 | L-serine + NADP+ | Saccharomyces cerevisiae | - |
2-aminomalonate semialdehyde + NADPH | - |
? | |
1.1.1.276 | L-serine + NADP+ | Saccharomyces cerevisiae YMR226C | - |
2-aminomalonate semialdehyde + NADPH | - |
? | |
1.1.1.276 | L-serine + NADP+ | Escherichia coli YdfG | - |
2-aminomalonate semialdehyde + NADPH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.276 | Escherichia coli | - |
YdfG | - |
1.1.1.276 | Escherichia coli YdfG | - |
YdfG | - |
1.1.1.276 | Saccharomyces cerevisiae | - |
YMR226C | - |
1.1.1.276 | Saccharomyces cerevisiae YMR226C | - |
YMR226C | - |
1.1.1.381 | Escherichia coli | P39831 | - |
- |
1.1.1.381 | Saccharomyces cerevisiae | Q05016 | - |
- |
1.1.1.381 | Saccharomyces cerevisiae ATCC 204508 | Q05016 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.276 | recombinant from overproducing Escherichia coli, to homogeneity | Escherichia coli |
1.1.1.276 | recombinant from overproducing Escherichia coli, to homogeneity | Saccharomyces cerevisiae |
1.1.1.381 | - |
Escherichia coli |
1.1.1.381 | - |
Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.276 | 2.4 | - |
purified recombinant enzyme | Escherichia coli |
1.1.1.276 | 6 | - |
purified recombinant enzyme | Saccharomyces cerevisiae |
1.1.1.381 | 2.4 | - |
pH 9.0, 30°C | Escherichia coli |
1.1.1.381 | 6 | - |
pH 9.0, 30°C | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.276 | 2-methyl-DL-serine + NADP+ | 59% activity compared to L-serine | Escherichia coli | 2-amino-2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
1.1.1.276 | 2-methyl-DL-serine + NADP+ | 21% activity compared to L-serine | Saccharomyces cerevisiae | 2-amino-2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
1.1.1.276 | 3-hydroxypropanoate + NADP+ | 23% activity compared to L-serine | Escherichia coli | 3-oxopropanoate + NADPH | - |
? | |
1.1.1.276 | 3-hydroxypropanoate + NADP+ | 13% activity compared to L-serine | Saccharomyces cerevisiae | 3-oxopropanoate + NADPH | - |
? | |
1.1.1.276 | D-3-hydroxybutyrate + NADP+ | 1% activity compared to L-serine | Escherichia coli | 3-oxobutyrate + NADPH | - |
? | |
1.1.1.276 | D-3-hydroxybutyrate + NADP+ | 12% activity compared to L-serine | Saccharomyces cerevisiae | 3-oxobutyrate + NADPH | - |
? | |
1.1.1.276 | D-3-hydroxyisobutyrate + NADP+ | 90% activity compared to L-serine | Escherichia coli | 2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
1.1.1.276 | D-3-hydroxyisobutyrate + NADP+ | 78% activity compared to L-serine | Saccharomyces cerevisiae | 2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
1.1.1.276 | D-glycerate + NADP+ | 48% activity compared to L-serine | Escherichia coli | ? | - |
? | |
1.1.1.276 | D-glycerate + NADP+ | 6% activity compared to L-serine | Saccharomyces cerevisiae | ? | - |
? | |
1.1.1.276 | D-serine + NADP+ | 83% activity compared to L-serine | Escherichia coli | 2-aminomalonate semialdehyde + NADPH | - |
? | |
1.1.1.276 | D-serine + NADP+ | 71% activity compared to L-serine | Saccharomyces cerevisiae | 2-aminomalonate semialdehyde + NADPH | - |
? | |
1.1.1.276 | D-serine + NADP+ | 71% activity compared to L-serine | Saccharomyces cerevisiae YMR226C | 2-aminomalonate semialdehyde + NADPH | - |
? | |
1.1.1.276 | D-serine + NADP+ | 83% activity compared to L-serine | Escherichia coli YdfG | 2-aminomalonate semialdehyde + NADPH | - |
? | |
1.1.1.276 | D-threonine + NADP+ | 156% activity compared to L-serine | Escherichia coli | D-2-amino-3-oxobutanoic acid + NADPH | - |
? | |
1.1.1.276 | D-threonine + NADP+ | 478% activity compared to L-serine | Saccharomyces cerevisiae | D-2-amino-3-oxobutanoic acid + NADPH | - |
? | |
1.1.1.276 | L-3-hydroxybutyrate + NADP+ | 85% activity compared to L-serine | Saccharomyces cerevisiae | 3-oxobutyrate + NADPH | - |
? | |
1.1.1.276 | L-3-hydroxybutyrate + NADP+ | 9% activity compared to L-serine | Escherichia coli | 3-oxobutyrate + NADPH | - |
? | |
1.1.1.276 | L-3-hydroxyisobutyrate + NADP+ | 96% activity compared to L-serine | Escherichia coli | 2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
1.1.1.276 | L-3-hydroxyisobutyrate + NADP+ | 182% activity compared to L-serine | Saccharomyces cerevisiae | 2-methyl-3-oxopropanoic acid + NADPH | - |
? | |
1.1.1.276 | L-allo-threonine + NADP+ | best substrate | Escherichia coli | L-2-amino-3-ketobutyrate + NADPH | L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone | ? | |
1.1.1.276 | L-allo-threonine + NADP+ | best substrate | Saccharomyces cerevisiae | L-2-amino-3-ketobutyrate + NADPH | L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone | ? | |
1.1.1.276 | L-allo-threonine + NADP+ | 137% activity compared to L-serine | Escherichia coli | L-2-amino-3-ketobutyrate + NADPH | L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone | ? | |
1.1.1.276 | L-allo-threonine + NADP+ | 397% activity compared to L-serine | Saccharomyces cerevisiae | L-2-amino-3-ketobutyrate + NADPH | L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone | ? | |
1.1.1.276 | L-glycerate + NADP+ | 28% activity compared to L-serine | Escherichia coli | ? | - |
? | |
1.1.1.276 | L-glycerate + NADP+ | 5% activity compared to L-serine | Saccharomyces cerevisiae | ? | - |
? | |
1.1.1.276 | L-glycerate + NADP+ | 5% activity compared to L-serine | Saccharomyces cerevisiae YMR226C | ? | - |
? | |
1.1.1.276 | L-glycerate + NADP+ | 28% activity compared to L-serine | Escherichia coli YdfG | ? | - |
? | |
1.1.1.276 | L-serine + NADP+ | - |
Escherichia coli | 2-aminomalonate semialdehyde + NADPH | - |
? | |
1.1.1.276 | L-serine + NADP+ | - |
Saccharomyces cerevisiae | 2-aminomalonate semialdehyde + NADPH | - |
? | |
1.1.1.276 | L-serine + NADP+ | - |
Saccharomyces cerevisiae YMR226C | 2-aminomalonate semialdehyde + NADPH | - |
? | |
1.1.1.276 | L-serine + NADP+ | - |
Escherichia coli YdfG | 2-aminomalonate semialdehyde + NADPH | - |
? | |
1.1.1.276 | additional information | no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol | Escherichia coli | ? | - |
? | |
1.1.1.276 | additional information | no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol | Saccharomyces cerevisiae | ? | - |
? | |
1.1.1.276 | additional information | L-threonine, D-allo-threonine are poor substrates | Escherichia coli | ? | - |
? | |
1.1.1.276 | additional information | L-threonine, D-allo-threonine are poor substrates | Saccharomyces cerevisiae | ? | - |
? | |
1.1.1.276 | additional information | no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol | Saccharomyces cerevisiae YMR226C | ? | - |
? | |
1.1.1.276 | additional information | L-threonine, D-allo-threonine are poor substrates | Saccharomyces cerevisiae YMR226C | ? | - |
? | |
1.1.1.276 | additional information | no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol | Escherichia coli YdfG | ? | - |
? | |
1.1.1.276 | additional information | L-threonine, D-allo-threonine are poor substrates | Escherichia coli YdfG | ? | - |
? | |
1.1.1.381 | D-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
1.1.1.381 | D-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 31% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
1.1.1.381 | D-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 1% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
1.1.1.381 | D-glycerate + NADP+ | the Vmax/KM value is 20% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
1.1.1.381 | D-serine + NADP+ | the Vmax/KM value is 15% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
1.1.1.381 | D-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
1.1.1.381 | D-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
1.1.1.381 | D-threonine + NADP+ | the Vmax/KM value is 55% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
1.1.1.381 | D-threonine + NADP+ | the Vmax/KM value is 55% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
1.1.1.381 | D-threonine + NADP+ | the Vmax/KM value is 55% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
1.1.1.381 | L-3-hydroxybutyrate + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
1.1.1.381 | L-3-hydroxybutyrate + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
1.1.1.381 | L-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 3.8% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
1.1.1.381 | L-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 34% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
1.1.1.381 | L-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 3.8% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
1.1.1.381 | L-allo-threonine + NADP+ | highest Vmax/Km value of all substrates tested. The hydroxyl group of L-allo-threonine is oxidized by the enzymes to yield L-2-amino-3-ketobutyrate, which is spontaneously decarboxylated into aminoacetone | Escherichia coli | aminoacetone + CO2 + NADPH + H+ | - |
? | |
1.1.1.381 | L-allo-threonine + NADP+ | highest Vmax/Km value of all substrates tested. The hydroxyl group of L-allo-threonine is oxidized by the enzymes to yield L-2-amino-3-oxobutyrate, which is spontaneously decarboxylated into aminoacetone | Saccharomyces cerevisiae | aminoacetone + CO2 + NADPH + H+ | - |
? | |
1.1.1.381 | L-glycerate + NADP+ | the Vmax/KM value is 18% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
1.1.1.381 | L-serine + NADP+ | the Vmax/KM value is 53% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
1.1.1.381 | L-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.276 | tetramer | 4 * 27000, recombinant enzyme, SDS-PAGE | Escherichia coli |
1.1.1.276 | tetramer | 4 * 29000, recombinant enzyme, SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.381 | ydfG | - |
Escherichia coli |
1.1.1.381 | YMR226c | - |
Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.381 | 30 | - |
assay at | Escherichia coli |
1.1.1.381 | 30 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.276 | 40 | - |
10 min, 0.1 M potassium phosphate, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, stable | Saccharomyces cerevisiae |
1.1.1.276 | 55 | - |
10 min, 0.1 M potassium phosphate, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, stable | Escherichia coli |
1.1.1.381 | 40 | - |
when heated for 10 min in 0.1 M potassium phosphate buffer (pH 7.4) containing 0.01% 2-mercaptoethanol and 10% glycerol, the enzyme is stable at up to 40°C | Saccharomyces cerevisiae |
1.1.1.381 | 55 | - |
when heated for 10 min in 0.1 M potassium phosphate buffer (pH 7.4) containing 0.01% 2-mercaptoethanol and 10% glycerol, the enzyme is stable at up to 55°C | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.276 | additional information | - |
additional information | substrate specificity | Escherichia coli | |
1.1.1.276 | additional information | - |
additional information | substrate specificity | Saccharomyces cerevisiae | |
1.1.1.276 | 4.2 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.276 | 5.76 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.276 | 11 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.276 | 43.8 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 1.17 | - |
L-Glycerate | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 2.01 | - |
D-glycerate | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 3.5 | - |
D-serine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 3.78 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 4.03 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 4.2 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 5.76 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 6.55 | - |
D-threonine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 7.84 | - |
D-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 8.61 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 9.38 | - |
L-3-hydroxybutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 11 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 20.1 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 43.8 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 52.8 | - |
D-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.276 | 8.5 | - |
oxidation of L-serine | Escherichia coli |
1.1.1.276 | 8.5 | - |
oxidation of L-serine | Saccharomyces cerevisiae |
1.1.1.381 | 8.5 | - |
oxidation of L-serine | Escherichia coli |
1.1.1.381 | 8.5 | - |
oxidation of L-serine | Saccharomyces cerevisiae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.276 | 6.5 | 10 | 10 min, 40°C, stable | Escherichia coli |
1.1.1.276 | 7.5 | 10.5 | 10 min, 40°C, stable | Saccharomyces cerevisiae |
1.1.1.381 | 6.5 | 10 | most stable over the pH-range 6.5 to 10.0 | Escherichia coli |
1.1.1.381 | 7.5 | 10.5 | most stable over the pH-range 6.5 to 10.0 | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.276 | NADP+ | dependent on | Escherichia coli | |
1.1.1.276 | NADP+ | dependent on | Saccharomyces cerevisiae | |
1.1.1.276 | NADP+ | NAD+ cannot replace NADP+ | Escherichia coli | |
1.1.1.276 | NADP+ | NAD+ cannot replace NADP+ | Saccharomyces cerevisiae | |
1.1.1.381 | NADP+ | no activity with NAD+ | Escherichia coli | |
1.1.1.381 | NADP+ | no activity with NAD+ | Saccharomyces cerevisiae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.381 | 0.036 | - |
L-Glycerate | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 0.04 | - |
D-glycerate | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 0.05 | - |
D-serine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 0.062 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 0.067 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 0.099 | - |
L-3-hydroxybutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 0.105 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 0.109 | - |
D-threonine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 0.11 | - |
D-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 0.116 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 0.151 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 0.199 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
1.1.1.381 | 0.558 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 7.5 | - |
D-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
1.1.1.381 | 14.6 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae |