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Literature summary extracted from

  • Fujisawa, H.; Nagata, S.; Misono, H.
    Characterization of short-chain dehydrogenase/reductase homologues of Escherichia coli (YdfG) and Saccharomyces cerevisiae (YMR226C) (2003), Biochim. Biophys. Acta, 1645, 89-94.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.276 overexpression in Escherichia coli JM109 Escherichia coli
1.1.1.276 overexpression in Escherichia coli JM109 Saccharomyces cerevisiae
1.1.1.381 expression in Escherichia coli JM109 Escherichia coli
1.1.1.381 expression in Escherichia coli JM109 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.276 additional information
-
additional information substrate specificity Escherichia coli
1.1.1.276 additional information
-
additional information substrate specificity Saccharomyces cerevisiae
1.1.1.276 0.5
-
NADP+ pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.276 0.54
-
NADP+ pH 9.0, 30°C Escherichia coli
1.1.1.276 3
-
L-allo-threonine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.276 29
-
L-allo-threonine pH 9.0, 30°C Escherichia coli
1.1.1.276 40
-
L-serine pH 9.0, 30°C Escherichia coli
1.1.1.276 95
-
L-serine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 0.54
-
NADP+ pH 9.0, 30°C Escherichia coli
1.1.1.381 3
-
L-allo-threonine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 7
-
D-threonine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 29
-
L-allo-threonine pH 9.0, 30°C Escherichia coli
1.1.1.381 33
-
L-Glycerate pH 9.0, 30°C Escherichia coli
1.1.1.381 36
-
L-3-hydroxyisobutyrate pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 40
-
L-serine pH 9.0, 30°C Escherichia coli
1.1.1.381 50
-
D-glycerate pH 9.0, 30°C Escherichia coli
1.1.1.381 57
-
D-3-hydroxyisobutyrate pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 60
-
D-threonine pH 9.0, 30°C Escherichia coli
1.1.1.381 60
-
L-3-hydroxyisobutyrate pH 9.0, 30°C Escherichia coli
1.1.1.381 61
-
D-3-hydroxyisobutyrate pH 9.0, 30°C Escherichia coli
1.1.1.381 69
-
D-serine pH 9.0, 30°C Escherichia coli
1.1.1.381 71
-
D-serine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 95
-
L-serine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 95
-
L-3-hydroxybutyrate pH 9.0, 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.276 27000
-
4 * 27000, recombinant enzyme, SDS-PAGE Escherichia coli
1.1.1.276 29000
-
4 * 29000, recombinant enzyme, SDS-PAGE Saccharomyces cerevisiae
1.1.1.276 105000
-
recombinant enzyme, gel filtration Escherichia coli
1.1.1.276 110000
-
recombinant enzyme, gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.276 L-serine + NADP+ Escherichia coli
-
2-aminomalonate semialdehyde + NADPH
-
?
1.1.1.276 L-serine + NADP+ Saccharomyces cerevisiae
-
2-aminomalonate semialdehyde + NADPH
-
?
1.1.1.276 L-serine + NADP+ Saccharomyces cerevisiae YMR226C
-
2-aminomalonate semialdehyde + NADPH
-
?
1.1.1.276 L-serine + NADP+ Escherichia coli YdfG
-
2-aminomalonate semialdehyde + NADPH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.276 Escherichia coli
-
YdfG
-
1.1.1.276 Escherichia coli YdfG
-
YdfG
-
1.1.1.276 Saccharomyces cerevisiae
-
YMR226C
-
1.1.1.276 Saccharomyces cerevisiae YMR226C
-
YMR226C
-
1.1.1.381 Escherichia coli P39831
-
-
1.1.1.381 Saccharomyces cerevisiae Q05016
-
-
1.1.1.381 Saccharomyces cerevisiae ATCC 204508 Q05016
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.276 recombinant from overproducing Escherichia coli, to homogeneity Escherichia coli
1.1.1.276 recombinant from overproducing Escherichia coli, to homogeneity Saccharomyces cerevisiae
1.1.1.381
-
Escherichia coli
1.1.1.381
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.276 2.4
-
purified recombinant enzyme Escherichia coli
1.1.1.276 6
-
purified recombinant enzyme Saccharomyces cerevisiae
1.1.1.381 2.4
-
pH 9.0, 30°C Escherichia coli
1.1.1.381 6
-
pH 9.0, 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.276 2-methyl-DL-serine + NADP+ 59% activity compared to L-serine Escherichia coli 2-amino-2-methyl-3-oxopropanoic acid + NADPH
-
?
1.1.1.276 2-methyl-DL-serine + NADP+ 21% activity compared to L-serine Saccharomyces cerevisiae 2-amino-2-methyl-3-oxopropanoic acid + NADPH
-
?
1.1.1.276 3-hydroxypropanoate + NADP+ 23% activity compared to L-serine Escherichia coli 3-oxopropanoate + NADPH
-
?
1.1.1.276 3-hydroxypropanoate + NADP+ 13% activity compared to L-serine Saccharomyces cerevisiae 3-oxopropanoate + NADPH
-
?
1.1.1.276 D-3-hydroxybutyrate + NADP+ 1% activity compared to L-serine Escherichia coli 3-oxobutyrate + NADPH
-
?
1.1.1.276 D-3-hydroxybutyrate + NADP+ 12% activity compared to L-serine Saccharomyces cerevisiae 3-oxobutyrate + NADPH
-
?
1.1.1.276 D-3-hydroxyisobutyrate + NADP+ 90% activity compared to L-serine Escherichia coli 2-methyl-3-oxopropanoic acid + NADPH
-
?
1.1.1.276 D-3-hydroxyisobutyrate + NADP+ 78% activity compared to L-serine Saccharomyces cerevisiae 2-methyl-3-oxopropanoic acid + NADPH
-
?
1.1.1.276 D-glycerate + NADP+ 48% activity compared to L-serine Escherichia coli ?
-
?
1.1.1.276 D-glycerate + NADP+ 6% activity compared to L-serine Saccharomyces cerevisiae ?
-
?
1.1.1.276 D-serine + NADP+ 83% activity compared to L-serine Escherichia coli 2-aminomalonate semialdehyde + NADPH
-
?
1.1.1.276 D-serine + NADP+ 71% activity compared to L-serine Saccharomyces cerevisiae 2-aminomalonate semialdehyde + NADPH
-
?
1.1.1.276 D-serine + NADP+ 71% activity compared to L-serine Saccharomyces cerevisiae YMR226C 2-aminomalonate semialdehyde + NADPH
-
?
1.1.1.276 D-serine + NADP+ 83% activity compared to L-serine Escherichia coli YdfG 2-aminomalonate semialdehyde + NADPH
-
?
1.1.1.276 D-threonine + NADP+ 156% activity compared to L-serine Escherichia coli D-2-amino-3-oxobutanoic acid + NADPH
-
?
1.1.1.276 D-threonine + NADP+ 478% activity compared to L-serine Saccharomyces cerevisiae D-2-amino-3-oxobutanoic acid + NADPH
-
?
1.1.1.276 L-3-hydroxybutyrate + NADP+ 85% activity compared to L-serine Saccharomyces cerevisiae 3-oxobutyrate + NADPH
-
?
1.1.1.276 L-3-hydroxybutyrate + NADP+ 9% activity compared to L-serine Escherichia coli 3-oxobutyrate + NADPH
-
?
1.1.1.276 L-3-hydroxyisobutyrate + NADP+ 96% activity compared to L-serine Escherichia coli 2-methyl-3-oxopropanoic acid + NADPH
-
?
1.1.1.276 L-3-hydroxyisobutyrate + NADP+ 182% activity compared to L-serine Saccharomyces cerevisiae 2-methyl-3-oxopropanoic acid + NADPH
-
?
1.1.1.276 L-allo-threonine + NADP+ best substrate Escherichia coli L-2-amino-3-ketobutyrate + NADPH L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone ?
1.1.1.276 L-allo-threonine + NADP+ best substrate Saccharomyces cerevisiae L-2-amino-3-ketobutyrate + NADPH L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone ?
1.1.1.276 L-allo-threonine + NADP+ 137% activity compared to L-serine Escherichia coli L-2-amino-3-ketobutyrate + NADPH L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone ?
1.1.1.276 L-allo-threonine + NADP+ 397% activity compared to L-serine Saccharomyces cerevisiae L-2-amino-3-ketobutyrate + NADPH L-2-amino-3-ketobutyrate spontaneously decarboxylates into aminoacetone ?
1.1.1.276 L-glycerate + NADP+ 28% activity compared to L-serine Escherichia coli ?
-
?
1.1.1.276 L-glycerate + NADP+ 5% activity compared to L-serine Saccharomyces cerevisiae ?
-
?
1.1.1.276 L-glycerate + NADP+ 5% activity compared to L-serine Saccharomyces cerevisiae YMR226C ?
-
?
1.1.1.276 L-glycerate + NADP+ 28% activity compared to L-serine Escherichia coli YdfG ?
-
?
1.1.1.276 L-serine + NADP+
-
Escherichia coli 2-aminomalonate semialdehyde + NADPH
-
?
1.1.1.276 L-serine + NADP+
-
Saccharomyces cerevisiae 2-aminomalonate semialdehyde + NADPH
-
?
1.1.1.276 L-serine + NADP+
-
Saccharomyces cerevisiae YMR226C 2-aminomalonate semialdehyde + NADPH
-
?
1.1.1.276 L-serine + NADP+
-
Escherichia coli YdfG 2-aminomalonate semialdehyde + NADPH
-
?
1.1.1.276 additional information no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol Escherichia coli ?
-
?
1.1.1.276 additional information no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol Saccharomyces cerevisiae ?
-
?
1.1.1.276 additional information L-threonine, D-allo-threonine are poor substrates Escherichia coli ?
-
?
1.1.1.276 additional information L-threonine, D-allo-threonine are poor substrates Saccharomyces cerevisiae ?
-
?
1.1.1.276 additional information no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol Saccharomyces cerevisiae YMR226C ?
-
?
1.1.1.276 additional information L-threonine, D-allo-threonine are poor substrates Saccharomyces cerevisiae YMR226C ?
-
?
1.1.1.276 additional information no activity with DL-homoserine, O-methyl-L-serine, N-acetyl-DL-serine, DL-isoserine, DL-threo-3-phenylserine, malonate, DL-malate, DL-lactate, DL-tartrate, D-gluconate, citrate, isocitrate, glycolate, glycerol Escherichia coli YdfG ?
-
?
1.1.1.276 additional information L-threonine, D-allo-threonine are poor substrates Escherichia coli YdfG ?
-
?
1.1.1.381 D-3-hydroxyisobutyrate + NADP+ the Vmax/KM value is 1% compared to L-allo-threonine Saccharomyces cerevisiae ?
-
?
1.1.1.381 D-3-hydroxyisobutyrate + NADP+ the Vmax/KM value is 31% compared to L-allo-threonine Escherichia coli ?
-
?
1.1.1.381 D-3-hydroxyisobutyrate + NADP+ the Vmax/KM value is 1% compared to L-allo-threonine Saccharomyces cerevisiae ATCC 204508 ?
-
?
1.1.1.381 D-glycerate + NADP+ the Vmax/KM value is 20% compared to L-allo-threonine Escherichia coli ?
-
?
1.1.1.381 D-serine + NADP+ the Vmax/KM value is 15% compared to L-allo-threonine Escherichia coli ?
-
?
1.1.1.381 D-serine + NADP+ the Vmax/KM value is less than 1% compared to L-allo-threonine Saccharomyces cerevisiae ?
-
?
1.1.1.381 D-serine + NADP+ the Vmax/KM value is less than 1% compared to L-allo-threonine Saccharomyces cerevisiae ATCC 204508 ?
-
?
1.1.1.381 D-threonine + NADP+ the Vmax/KM value is 55% compared to L-allo-threonine Escherichia coli ?
-
?
1.1.1.381 D-threonine + NADP+ the Vmax/KM value is 55% compared to L-allo-threonine Saccharomyces cerevisiae ?
-
?
1.1.1.381 D-threonine + NADP+ the Vmax/KM value is 55% compared to L-allo-threonine Saccharomyces cerevisiae ATCC 204508 ?
-
?
1.1.1.381 L-3-hydroxybutyrate + NADP+ the Vmax/KM value is less than 1% compared to L-allo-threonine Saccharomyces cerevisiae ?
-
?
1.1.1.381 L-3-hydroxybutyrate + NADP+ the Vmax/KM value is less than 1% compared to L-allo-threonine Saccharomyces cerevisiae ATCC 204508 ?
-
?
1.1.1.381 L-3-hydroxyisobutyrate + NADP+ the Vmax/KM value is 3.8% compared to L-allo-threonine Saccharomyces cerevisiae ?
-
?
1.1.1.381 L-3-hydroxyisobutyrate + NADP+ the Vmax/KM value is 34% compared to L-allo-threonine Escherichia coli ?
-
?
1.1.1.381 L-3-hydroxyisobutyrate + NADP+ the Vmax/KM value is 3.8% compared to L-allo-threonine Saccharomyces cerevisiae ATCC 204508 ?
-
?
1.1.1.381 L-allo-threonine + NADP+ highest Vmax/Km value of all substrates tested. The hydroxyl group of L-allo-threonine is oxidized by the enzymes to yield L-2-amino-3-ketobutyrate, which is spontaneously decarboxylated into aminoacetone Escherichia coli aminoacetone + CO2 + NADPH + H+
-
?
1.1.1.381 L-allo-threonine + NADP+ highest Vmax/Km value of all substrates tested. The hydroxyl group of L-allo-threonine is oxidized by the enzymes to yield L-2-amino-3-oxobutyrate, which is spontaneously decarboxylated into aminoacetone Saccharomyces cerevisiae aminoacetone + CO2 + NADPH + H+
-
?
1.1.1.381 L-glycerate + NADP+ the Vmax/KM value is 18% compared to L-allo-threonine Escherichia coli ?
-
?
1.1.1.381 L-serine + NADP+ the Vmax/KM value is 53% compared to L-allo-threonine Escherichia coli ?
-
?
1.1.1.381 L-serine + NADP+ the Vmax/KM value is less than 1% compared to L-allo-threonine Saccharomyces cerevisiae ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.276 tetramer 4 * 27000, recombinant enzyme, SDS-PAGE Escherichia coli
1.1.1.276 tetramer 4 * 29000, recombinant enzyme, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.381 ydfG
-
Escherichia coli
1.1.1.381 YMR226c
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.381 30
-
assay at Escherichia coli
1.1.1.381 30
-
assay at Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.276 40
-
10 min, 0.1 M potassium phosphate, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, stable Saccharomyces cerevisiae
1.1.1.276 55
-
10 min, 0.1 M potassium phosphate, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, stable Escherichia coli
1.1.1.381 40
-
when heated for 10 min in 0.1 M potassium phosphate buffer (pH 7.4) containing 0.01% 2-mercaptoethanol and 10% glycerol, the enzyme is stable at up to 40°C Saccharomyces cerevisiae
1.1.1.381 55
-
when heated for 10 min in 0.1 M potassium phosphate buffer (pH 7.4) containing 0.01% 2-mercaptoethanol and 10% glycerol, the enzyme is stable at up to 55°C Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.276 additional information
-
additional information substrate specificity Escherichia coli
1.1.1.276 additional information
-
additional information substrate specificity Saccharomyces cerevisiae
1.1.1.276 4.2
-
L-serine pH 9.0, 30°C Escherichia coli
1.1.1.276 5.76
-
L-allo-threonine pH 9.0, 30°C Escherichia coli
1.1.1.276 11
-
L-serine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.276 43.8
-
L-allo-threonine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 1.17
-
L-Glycerate pH 9.0, 30°C Escherichia coli
1.1.1.381 2.01
-
D-glycerate pH 9.0, 30°C Escherichia coli
1.1.1.381 3.5
-
D-serine pH 9.0, 30°C Escherichia coli
1.1.1.381 3.78
-
D-3-hydroxyisobutyrate pH 9.0, 30°C Escherichia coli
1.1.1.381 4.03
-
L-3-hydroxyisobutyrate pH 9.0, 30°C Escherichia coli
1.1.1.381 4.2
-
L-serine pH 9.0, 30°C Escherichia coli
1.1.1.381 5.76
-
L-allo-threonine pH 9.0, 30°C Escherichia coli
1.1.1.381 6.55
-
D-threonine pH 9.0, 30°C Escherichia coli
1.1.1.381 7.84
-
D-serine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 8.61
-
D-3-hydroxyisobutyrate pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 9.38
-
L-3-hydroxybutyrate pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 11
-
L-serine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 20.1
-
L-3-hydroxyisobutyrate pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 43.8
-
L-allo-threonine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 52.8
-
D-threonine pH 9.0, 30°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.276 8.5
-
oxidation of L-serine Escherichia coli
1.1.1.276 8.5
-
oxidation of L-serine Saccharomyces cerevisiae
1.1.1.381 8.5
-
oxidation of L-serine Escherichia coli
1.1.1.381 8.5
-
oxidation of L-serine Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.276 6.5 10 10 min, 40°C, stable Escherichia coli
1.1.1.276 7.5 10.5 10 min, 40°C, stable Saccharomyces cerevisiae
1.1.1.381 6.5 10 most stable over the pH-range 6.5 to 10.0 Escherichia coli
1.1.1.381 7.5 10.5 most stable over the pH-range 6.5 to 10.0 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.276 NADP+ dependent on Escherichia coli
1.1.1.276 NADP+ dependent on Saccharomyces cerevisiae
1.1.1.276 NADP+ NAD+ cannot replace NADP+ Escherichia coli
1.1.1.276 NADP+ NAD+ cannot replace NADP+ Saccharomyces cerevisiae
1.1.1.381 NADP+ no activity with NAD+ Escherichia coli
1.1.1.381 NADP+ no activity with NAD+ Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.381 0.036
-
L-Glycerate pH 9.0, 30°C Escherichia coli
1.1.1.381 0.04
-
D-glycerate pH 9.0, 30°C Escherichia coli
1.1.1.381 0.05
-
D-serine pH 9.0, 30°C Escherichia coli
1.1.1.381 0.062
-
D-3-hydroxyisobutyrate pH 9.0, 30°C Escherichia coli
1.1.1.381 0.067
-
L-3-hydroxyisobutyrate pH 9.0, 30°C Escherichia coli
1.1.1.381 0.099
-
L-3-hydroxybutyrate pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 0.105
-
L-serine pH 9.0, 30°C Escherichia coli
1.1.1.381 0.109
-
D-threonine pH 9.0, 30°C Escherichia coli
1.1.1.381 0.11
-
D-serine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 0.116
-
L-serine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 0.151
-
D-3-hydroxyisobutyrate pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 0.199
-
L-allo-threonine pH 9.0, 30°C Escherichia coli
1.1.1.381 0.558
-
L-3-hydroxyisobutyrate pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 7.5
-
D-threonine pH 9.0, 30°C Saccharomyces cerevisiae
1.1.1.381 14.6
-
L-allo-threonine pH 9.0, 30°C Saccharomyces cerevisiae