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Literature summary extracted from

  • Dean, A.M.; Dvorak, L.
    The role of glutamate 87 in the kinetic mechanism of Thermus thermophilus isopropylmalate dehydrogenase (1995), Protein Sci., 4, 2156-2167.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.85 E87G mutation does not affect the dissociation constant from the binary complex, but does greatly increase the KM-values Thermus thermophilus
1.1.1.85 E87Q mutation does not affect the dissociation constant from the binary complex, but does greatly increase the KM-values Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.85 isocitrate
-
 Thermus thermophilus
1.1.1.85 NADH NADH always produces competitive inhibition patterns with respect to NAD and noncompetitive inhibition patterns with respect to the substrates Thermus thermophilus
1.1.1.85 Tartrate
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.000017
-
 isopropylmalate pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme Thermus thermophilus
1.1.1.85 0.00048
-
 isopropylmalate pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87G Thermus thermophilus
1.1.1.85 0.00298
-
 isopropylmalate pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme Thermus thermophilus
1.1.1.85 0.00321
-
 NAD+ pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme Thermus thermophilus
1.1.1.85 0.0053
-
 isopropylmalate pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87Q Thermus thermophilus
1.1.1.85 0.0326
-
 malate pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87Q Thermus thermophilus
1.1.1.85 0.0407
-
 Tartrate pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme Thermus thermophilus
1.1.1.85 0.0454
-
 NAD+ pH 7.3, 21°C, reaction with tartrate, wild-type enzym Thermus thermophilus
1.1.1.85 0.1065
-
 NAD+ pH 7.3, 21°C, reaction with malate, wild-type enzyme Thermus thermophilus
1.1.1.85 0.131
-
 NAD+ pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87G Thermus thermophilus
1.1.1.85 0.181
-
 NAD+ pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87Q Thermus thermophilus
1.1.1.85 0.386
-
 NAD+ pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87G Thermus thermophilus
1.1.1.85 0.761
-
 Tartrate pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87Q Thermus thermophilus
1.1.1.85 0.778
-
 NAD+ pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87Q Thermus thermophilus
1.1.1.85 0.817
-
 Tartrate pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme Thermus thermophilus
1.1.1.85 1.21
-
 Tartrate pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87G Thermus thermophilus
1.1.1.85 1.51
-
 malate pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme Thermus thermophilus
1.1.1.85 1.573
-
 NADP+ pH 7.3, 21°C, reaction with tartrate, wild-type enzyme Thermus thermophilus
1.1.1.85 1.836
-
 NADP+ pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme Thermus thermophilus
1.1.1.85 1.853
-
 NAD+ pH 7.3, 21°C, reaction with malate, mutant enzyme E87Q Thermus thermophilus
1.1.1.85 2.584
-
 NAD+ pH 7.3, 21°C, reaction with malate, mutant enzyme E87G Thermus thermophilus
1.1.1.85 6.579
-
 NADP+ pH 7.3, 21°C, recation with malate, wild-type enzyme Thermus thermophilus
1.1.1.85 31.56
-
 malate pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme Thermus thermophilus
1.1.1.85 35.59
-
 malate pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87G Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Thermus thermophilus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ = (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ the oxidative decarboxylation of 2-hydroxy acid substrates by the enzyme occurs via a random kinetic mechanism with the formation of two abortive complexes: enzyme-NADH-substrate and enzyme-NADH-inhibitor Thermus thermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Thermus thermophilus 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
1.1.1.85 malate + NAD+
-
 Thermus thermophilus pyruvate + NADH + H+ + CO2
-
 ?
1.1.1.85 malate + NADP+
-
 Thermus thermophilus pyruvate + NADPH + CO2
-
 ?
1.1.1.85 tartrate + NAD+
-
 Thermus thermophilus ?
-
 ?
1.1.1.85 tartrate + NADP+
-
 Thermus thermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.85 dimer 2 * 35000-40000, SDS-PAGE Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.85 0.1
-
 Tartrate pH 7.3, 21°C, reaction with NADP+, wild-type enzyme Thermus thermophilus
1.1.1.85 0.1
-
 NADP+ pH 7.3, 21°C, reaction with tartrate, wild-type enzyme Thermus thermophilus
1.1.1.85 0.19
-
 Tartrate pH 7.3, 21°C, reaction with NAD+, wild-type enzyme Thermus thermophilus
1.1.1.85 0.19
-
 malate pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q Thermus thermophilus
1.1.1.85 0.19
-
 NAD+ pH 7.3, 21°C, reaction with tartrate, wild-type enzyme or reaction with malate, mutant enzyme E87Q Thermus thermophilus
1.1.1.85 0.2
-
 isopropylmalate pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G Thermus thermophilus
1.1.1.85 0.2
-
 NAD+ pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87G Thermus thermophilus
1.1.1.85 0.21
-
 isopropylmalate pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q Thermus thermophilus
1.1.1.85 0.21
-
 Tartrate pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G Thermus thermophilus
1.1.1.85 0.21
-
 NAD+ pH 7.3, 21°C, reaction with tartrate, mutant enzyme E87G Thermus thermophilus
1.1.1.85 0.21
-
 NAD+ pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87Q Thermus thermophilus
1.1.1.85 0.24
-
 isopropylmalate pH 7.3, 21°C, reaction with NAD+, wild-type enzyme Thermus thermophilus
1.1.1.85 0.24
-
 NAD+ pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme Thermus thermophilus
1.1.1.85 0.26
-
 Tartrate pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q Thermus thermophilus
1.1.1.85 0.26
-
 malate pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G Thermus thermophilus
1.1.1.85 0.26
-
 NAD+ pH 7.3, 21°C, reaction with malate, mutant enzyme E87G or reaction with tartrate, mutant enzyme E87Q Thermus thermophilus
1.1.1.85 1.66
-
 isopropylmalate pH 7.3, 21°C, reaction with NADP+, wild-type enzyme Thermus thermophilus
1.1.1.85 1.66
-
 NADP+ pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme Thermus thermophilus
1.1.1.85 4.69
-
 malate pH 7.3, 21°C, reaction with NADP+, wild-type enzyme Thermus thermophilus
1.1.1.85 4.69
-
 NADP+ pH 7.3, 21°C, reaction with malate, wild-type enzyme Thermus thermophilus
1.1.1.85 10.6
-
 malate pH 7.3, 21°C, reaction with NAD+, wild-type enzyme Thermus thermophilus
1.1.1.85 10.6
-
 NAD+ pH 7.3, 21°C, reaction with malate, wild-type enzyme Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Thermus thermophilus
1.1.1.85 NADP+
-
 Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.85 additional information
-
 additional information
-
 Thermus thermophilus