Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Durner, J.; Knoerzer, O.C.; Boeger, P.
    Ketol-acid reductoisomerase from barley (Hordeum vulgare). Purification, properties, and specific inhibition (1993), Plant Physiol., 103, 903-910.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.86 2-dimethylphosphinoyl-2-hydroxyacetic acid competitive, reversible Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.86 0.0043
-
 NADPH pH 7.5, 25°C Hordeum vulgare
1.1.1.86 0.011
-
 2-acetolactate pH 7.5, 25°C Hordeum vulgare
1.1.1.86 0.038
-
 2-aceto-2-hydroxybutyrate pH 7.5, 25°C Hordeum vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.86 Mg2+ required Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.86 59000
-
 2 * 59000, SDS-PAGE Hordeum vulgare
1.1.1.86 115000
-
 gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.86 2-acetolactate + NADPH Hordeum vulgare enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.86 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.86
-
 Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.86 shoot
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.86 3.04
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.86 2-aceto-2-hydroxybutyrate + NADPH + H+
-
 Hordeum vulgare 2,3-dihydroxy-3-methylvalerate + NADP+
-
 r
1.1.1.86 2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Hordeum vulgare 2,3-dihydroxy-3-methylbutanoate + NADP+
-
 r
1.1.1.86 NADPH + 2-acetolactate
-
 Hordeum vulgare NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.86 dimer 2 * 59000, SDS-PAGE Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.86 7.5
-
 2-acetolactate as substrate Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.86 NADPH
-
 Hordeum vulgare

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.86 0.00019
-
 2-dimethylphosphinoyl-2-hydroxyacetic acid pH 7.5, 25°C, with 2-aceto-2-hydroxybutyrate as substrate Hordeum vulgare
1.1.1.86 0.00046
-
 2-dimethylphosphinoyl-2-hydroxyacetic acid pH 7.5, 25°C, with 2-acetolactate as substrate Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.86 Hordeum vulgare chromatofocusing 4.7 4.6