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Literature summary extracted from

  • Matsushita, K.; Shinagawa, E.; Adachi, O.; Ameyama, M.
    Quinoprotein D-glucose dehydrogenase in Acinetobacetr calcoaceticus LMD 79.41: purification and characterization of the membrane-bound enzyme distinct from the soluble enzyme (1989), Antonie van Leeuwenhoek, 56, 63-72.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 0.0034
-
 ubiquinone Q6 membrane-bound enzyme form Acinetobacter calcoaceticus
1.1.5.2 0.0044
-
 ubiquinone Q9 membrane-bound enzyme form Acinetobacter calcoaceticus
1.1.5.2 0.012
-
 ubiquinone Q2 membrane-bound enzyme form Acinetobacter calcoaceticus
1.1.5.2 0.0177
-
 ubiquinone Q2 soluble enzyme form Acinetobacter calcoaceticus
1.1.5.2 0.074
-
 phenazine methosulfate membrane-bound enzyme form Acinetobacter calcoaceticus
1.1.5.2 0.148
-
 ubiquinone Q1 membrane-bound enzyme form Acinetobacter calcoaceticus
1.1.5.2 0.178
-
 ubiquinone Q1 soluble enzyme form Acinetobacter calcoaceticus
1.1.5.2 1.9
-
 phenazine methosulfate soluble enzyme form Acinetobacter calcoaceticus
1.1.5.2 4.2
-
 D-glucose membrane-bound enzyme form Acinetobacter calcoaceticus
1.1.5.2 24.5
-
 D-glucose soluble enzyme Acinetobacter calcoaceticus
1.1.5.2 26.7
-
 lactose soluble enzyme Acinetobacter calcoaceticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 membrane enzyme exists as a soluble form and a membrane-bound form Acinetobacter calcoaceticus 16020
-
1.1.5.2 soluble
-
 Acinetobacter calcoaceticus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.2 55000
-
 x * 55000, soluble enzyme SDS-PAGE Acinetobacter calcoaceticus
1.1.5.2 83000
-
 1 * 83000, membrane-bound enzyme form, mainly monomeric in detergent solution Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.2 D-glucose + pyrroloquinoline quinone Acinetobacter calcoaceticus membrane-bound enzyme functions by linking to the respiratory chain via ubiquinone D-glucono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 D-glucose + pyrroloquinoline quinone Acinetobacter calcoaceticus LMD 79.41 membrane-bound enzyme functions by linking to the respiratory chain via ubiquinone D-glucono-1,5-lactone + pyrroloquinoline quinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Acinetobacter calcoaceticus
-
 LMD 79.41
-
1.1.5.2 Acinetobacter calcoaceticus LMD 79.41
-
 LMD 79.41
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.2
-
 Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.2 570
-
 membrane bound enzyme form Acinetobacter calcoaceticus
1.1.5.2 2209
-
 soluble enzyme form Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 D-glucose + phenazine methosulfate
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + phenazine methosulfate
-
 Acinetobacter calcoaceticus LMD 79.41 D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + pyrroloquinoline quinone membrane-bound enzyme functions by linking to the respiratory chain via ubiquinone Acinetobacter calcoaceticus D-glucono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 D-glucose + pyrroloquinoline quinone membrane-bound enzyme functions by linking to the respiratory chain via ubiquinone Acinetobacter calcoaceticus LMD 79.41 D-glucono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 D-glucose + ubiquinone Q1
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone Q1
-
 Acinetobacter calcoaceticus LMD 79.41 D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone Q2
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone Q2
-
 Acinetobacter calcoaceticus LMD 79.41 D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone Q6
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone Q6
-
 Acinetobacter calcoaceticus LMD 79.41 D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone Q9
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ubiquinol Q9
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.2 ? x * 55000, soluble enzyme SDS-PAGE Acinetobacter calcoaceticus
1.1.5.2 monomer 1 * 83000, membrane-bound enzyme form, mainly monomeric in detergent solution Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 6.5
-
 soluble enzyme Acinetobacter calcoaceticus
1.1.5.2 8.5
-
 membrane-bound enzyme Acinetobacter calcoaceticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.5.2 7.5 9.5 pH 7.5: about 40% of maximal activity, pH 9.5: about 85% of maximal activity, membrane-bound enzyme, reaction with phenazine methosulfate, 2,6-dichlorophenol indophenol and pyrroloquinoline quinone Acinetobacter calcoaceticus