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Literature summary extracted from

  • Matsushita, K.; Ameyama, M.
    D-Glucose dehydrogenase from Pseudomonas fluorescens, membrane-bound (1982), Methods Enzymol., 89, 149-154.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.2 EDTA 3.3 mM, complete inhibition Pseudomonas fluorescens
1.1.5.2 p-benzoquinone 1.7 mM, complete inhibition Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 0.0097
-
 ubiquinone Q6 pH 8.8, 25°C Pseudomonas fluorescens
1.1.5.2 0.031
-
 ubiquinone Q4 pH 8.8, 25°C Pseudomonas fluorescens
1.1.5.2 0.06
-
 ubiquinone Q1 pH 8.8, 25°C Pseudomonas fluorescens
1.1.5.2 0.061
-
 ubiquinone Q2 pH 8.8, 25°C Pseudomonas fluorescens
1.1.5.2 0.13
-
 phenazine methosulfate pH 8.8, 25°C Pseudomonas fluorescens
1.1.5.2 0.47
-
 D-glucose pH 6.0, 25°C, reaction with 2,6-dichlorophenolindophenol Pseudomonas fluorescens
1.1.5.2 0.56
-
 N,N,N,N-tetramethyl-o-phenylenediamine pH 8.8, 25°C Pseudomonas fluorescens
1.1.5.2 0.69
-
 ferricyanide pH 8.8, 25°C Pseudomonas fluorescens
1.1.5.2 1.6
-
 2,6-dichlorophenolindophenol pH 8.8, 25°C Pseudomonas fluorescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 cytoplasmic membrane outer surface of the cytoplasmic membrane Pseudomonas fluorescens
-
-
1.1.5.2 membrane bound to Pseudomonas fluorescens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.2 87000
-
 1 * 87000, urea-SDS-PAGE Pseudomonas fluorescens
1.1.5.2 93000
-
 sucrose density gradient centrifugation Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.2
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.2 386
-
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 D-galactose + pyrroloquinoline quinone 6.5% of the activity with D-glucose Pseudomonas fluorescens D-galactono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 D-glucose + ferricyanide
-
 Pseudomonas fluorescens D-glucono-1,5-lactone + ferrocyanide
-
 ?
1.1.5.2 D-glucose + N,N,N,N-tetramethyl-o-phenylenediamine
-
 Pseudomonas fluorescens ?
-
 r
1.1.5.2 D-glucose + phenazine methosulfate
-
 Pseudomonas fluorescens D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone Q1
-
 Pseudomonas fluorescens D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone Q2
-
 Pseudomonas fluorescens D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone Q4
-
 Pseudomonas fluorescens D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone Q6
-
 Pseudomonas fluorescens D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-mannose + pyrroloquinoline quinone 8.6% of the activity with D-glucose Pseudomonas fluorescens D-mannono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 D-xylose + pyrroloquinoline quinone 13% of the activity with D-glucose Pseudomonas fluorescens D-xylono-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 L-arabinose + pyrroloquinoline quinone 2.8% of the activity with D-glucose Pseudomonas fluorescens L-arabino-1,5-lactone + pyrroloquinoline quinol
-
 ?
1.1.5.2 maltose + pyrroloquinoline quinone 3.2% of the activity with D-glucose Pseudomonas fluorescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.2 monomer 1 * 87000, urea-SDS-PAGE Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone prosthetic group Pseudomonas fluorescens