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Literature summary extracted from

  • Sode, K.; Ootera, T.; Shirahane, M.; Witarto, A.B.; Igarashi, S.; Yoshida, H.
    Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement (2000), Enzyme Microb. Technol., 26, 491-496.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.5.2 analysis application of mutant enzyme S231K as a glucose sensor constituent. The mutant has more than 8fold increase in its half-life during the thermal inactivation at 55 C compared with the wild-type enzyme and retains catalytic activity similar to the wild-type enzyme Acinetobacter calcoaceticus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.2 S231C increase in thermal stability Acinetobacter calcoaceticus
1.1.5.2 S231D increase in thermal stability Acinetobacter calcoaceticus
1.1.5.2 S231H increase in thermal stability Acinetobacter calcoaceticus
1.1.5.2 S231K more than 8fold increase in its half-life during the thermal inactivation at 55 C compared with the wild-type enzyme, retains catalytic activity similar to the wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 S231L increase in thermal stability Acinetobacter calcoaceticus
1.1.5.2 S231M increase in thermal stability Acinetobacter calcoaceticus
1.1.5.2 S231N increase in thermal stability Acinetobacter calcoaceticus
1.1.99.35 S231C substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 3%, D-mannose 8%, D-allose 46%, 3-O-methyl-D-glucose 76%, D-galactose 14%, D-xylose 8%, D-lactose 69%, D-maltose 69%, respectively Acinetobacter calcoaceticus
1.1.99.35 S231D substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 9%, D-allose 38%, 3-O-methyl-D-glucose 71%, D-galactose 12%, D-xylose 8%, D-lactose 54%, D-maltose 38%, respectively Acinetobacter calcoaceticus
1.1.99.35 S231H substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 2%, D-mannose 12%, D-allose 57%, 3-O-methyl-D-glucose 86%, D-galactose 17%, D-xylose 7%, D-lactose 56%, D-maltose 38%, respectively Acinetobacter calcoaceticus
1.1.99.35 S231K increase in thermal stability. Substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 10%, D-allose 43%, 3-O-methyl-D-glucose 82%, D-galactose 15%, D-xylose 5%, D-lactose 59%, D-maltose 70%, respectively Acinetobacter calcoaceticus
1.1.99.35 S231L substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 6%, D-mannose 13%, D-allose 62%, 3-O-methyl-D-glucose 105%, D-galactose 20%, D-xylose 12%, D-lactose 73%, D-maltose 76%, respectively Acinetobacter calcoaceticus
1.1.99.35 S231M substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 9%, D-allose 43%, 3-O-methyl-D-glucose 80%, D-galactose 10%, D-xylose 8%, D-lactose 56%, D-maltose 41%, respectively Acinetobacter calcoaceticus
1.1.99.35 S231N substrate specificity: D-glucose 100% activity, 2-deoxy-D-glucose 5%, D-mannose 12%, D-allose 61%, 3-O-methyl-D-glucose 109%, D-galactose 18%, D-xylose 15%, D-lactose 66%, D-maltose 51%, respectively Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.35 25
-
 D-glucose wild-type, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 27
-
 D-glucose mutant S231K, pH 7.0 Acinetobacter calcoaceticus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Acinetobacter calcoaceticus
-
-
-
1.1.99.35 Acinetobacter calcoaceticus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.35 2977
-
 wild-type, pH 7.0, at 50 mM D-glucose Acinetobacter calcoaceticus
1.1.99.35 3313
-
 mutant S231K, pH 7.0, at 100 mM D-glucose Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.35 2-deoxy-D-glucose + oxidized 2,6-dichlorophenolindophenol 4% of the activity with D-glucose Acinetobacter calcoaceticus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol 81% of the activity with D-glucose Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-allose + oxidized 2,6-dichlorophenolindophenol 47% of the activity with D-glucose Acinetobacter calcoaceticus D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-galactose + oxidized 2,6-dichlorophenolindophenol 11% of the activity with D-glucose Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-glucose + oxidized 2,6-dichlorophenolindophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-mannose + oxidized 2,6-dichlorophenolindophenol 13% of the activity with D-glucose Acinetobacter calcoaceticus D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-xylose + oxidized 2,6-dichlorophenolindophenol 7% of the activity with D-glucose Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 lactose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 maltose + oxidized 2,6-dichlorophenolindophenol 61% of the activity with D-glucose Acinetobacter calcoaceticus maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.35 55
-
 wild-type, 5 min, 50% loss of activity. Mutant S231K, 50% loss of activity after 41 min Acinetobacter calcoaceticus