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Literature summary extracted from

  • Oubrie, A.; Rozeboom, H.J.; Dijkstra, B.W.
    Active-site structure of the soluble quinoprotein glucose dehydrogenase complexed with methylhydrazine: A covalent cofactor-inhibitor complex (1999), Proc. Natl. Acad. Sci. USA, 96, 11787-11791.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.99.35 ternary complex of s-GDH with PQQ and methylhydrazine, at 1.5 A resolution. Formation of a covalent PQQ adduct in the active-site. The C5 carbonyl group of the cofactor is the most reactive moiety of PQQ. The binding of the cofactor to s-GDH is predominantly governed by polar interactions. The C2, C7, and C9 carboxyl groups of PQQ form salt bridges with Arg408, Lys377, and Arg406, respectively. The ortho-quinone O4 and O5 atoms are bound by Asn229 and Arg228, respectively. The N6, O7A, and O5 atoms of PQQ are ligands for the active-site calcium ion. The other calcium ligands are provided by the two main chain carbonyl oxygen atoms of Gly-247 and Pro-248 and two watermolecules Acinetobacter calcoaceticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.35 methylhydrazine competitive inhibitor Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 0.064
-
 N-methylphenazonium methyl sulfate pH 8.5, reaction with D-galactose Acinetobacter calcoaceticus
1.1.5.2 0.118
-
 L-arabinose pH 8.5, reaction with D-glucose Acinetobacter calcoaceticus
1.1.5.2 0.156
-
 N-methylphenazonium methyl sulfate pH 8.5, reaction with D-xylose Acinetobacter calcoaceticus
1.1.5.2 0.178
-
 N-methylphenazonium methyl sulfate pH 8.5, reaction with D-glucose Acinetobacter calcoaceticus
1.1.5.2 0.22
-
 N-methylphenazonium methyl sulfate pH 8.5, reaction with D-fucose Acinetobacter calcoaceticus
1.1.5.2 0.23
-
 D-glucose pH 8.5, reaction with 2,6-dichlorophenolindophenol as electron acceptor Acinetobacter calcoaceticus
1.1.5.2 0.268
-
 N-ethylphenazonium ethyl sulfate pH 8.5, reaction with D-xylose Acinetobacter calcoaceticus
1.1.5.2 0.278
-
 N-ethylphenazonium ethyl sulfate pH 8.5, reaction with D-glucose Acinetobacter calcoaceticus
1.1.5.2 0.291
-
 N-ethylphenazonium ethyl sulfate pH 8.5, reaction with D-fucose Acinetobacter calcoaceticus
1.1.5.2 0.292
-
 N-ethylphenazonium ethyl sulfate pH 8.5, reaction with L-arabinose Acinetobacter calcoaceticus
1.1.5.2 0.362
-
 N-methylphenazonium methyl sulfate pH 8.5, reaction with 2-deoxy-D-glucose Acinetobacter calcoaceticus
1.1.5.2 4
-
 D-glucose pH 8.5, reaction with N-methylphenazonium methyl sulfate or N-ethylphenazonium ethal sulfate as electron acceptor Acinetobacter calcoaceticus
1.1.5.2 5
-
 D-fucose pH 8.5, reaction with N-ethylphenazonium ethyl sulfate as electron acceptor Acinetobacter calcoaceticus
1.1.5.2 5.9
-
 D-glucose pH 8.5, reaction with Wurster‘ Blue as electron acceptor Acinetobacter calcoaceticus
1.1.5.2 7
-
 D-xylose pH 8.5, reaction with N-ethylphenazonium ethyl sulfate as electron acceptor Acinetobacter calcoaceticus
1.1.5.2 12
-
 D-xylose pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor Acinetobacter calcoaceticus
1.1.5.2 12
-
 D-fucose pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor Acinetobacter calcoaceticus
1.1.5.2 18
-
 L-arabinose pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor Acinetobacter calcoaceticus
1.1.5.2 19
-
 L-arabinose pH 8.5, reaction with N-ethylphenazonium ethyl sulfate as electron acceptor Acinetobacter calcoaceticus
1.1.5.2 19
-
 D-galactose pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor Acinetobacter calcoaceticus
1.1.5.2 22
-
 2-deoxy-D-glucose pH 8.5, reaction with N-methylphenazonium methyl sulfate as electron acceptor Acinetobacter calcoaceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.2 Mg2+ Mg2+ anchors pyrroloquinoline quinone cofactor to the enzyme protein and activates the bound cofactor Acinetobacter calcoaceticus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Acinetobacter calcoaceticus
-
-
-
1.1.99.35 Acinetobacter calcoaceticus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.2 612
-
-
 Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 2-deoxy-D-glucose + oxidized N-methylphenazonium methyl sulfate
-
 Acinetobacter calcoaceticus 2-deoxy-D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
 ?
1.1.5.2 D-fucose + N-ethylphenazonium ethyl sulfate
-
 Acinetobacter calcoaceticus 6-deoxy-D-galactono-1,5-lactone + ?
-
 ?
1.1.5.2 D-fucose + oxidized N-methylphenazonium methyl sulfate
-
 Acinetobacter calcoaceticus 6-deoxy-D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
 ?
1.1.5.2 D-galactose + oxidized N-methylphenazonium methyl sulfate
-
 Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
 ?
1.1.5.2 D-glucose + 2,6-dichlorophenol-indophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + N-ethylphenazonium ethyl sulfate
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + oxidized N-methylphenazonium methyl sulfate
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
 ?
1.1.5.2 D-xylose + N-ethylphenazonium ethyl sulfate
-
 Acinetobacter calcoaceticus D-xylono-1,5-lactone + ?
-
 ?
1.1.5.2 D-xylose + oxidized N-methylphenazonium methyl sulfate
-
 Acinetobacter calcoaceticus D-xylono-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
 ?
1.1.5.2 L-arabinose + N-ethylphenazonium ethyl sulfate
-
 Acinetobacter calcoaceticus L-arabino-1,5-lactone + ?
-
 ?
1.1.5.2 L-arabinose + oxidized N-methylphenazonium methyl sulfate
-
 Acinetobacter calcoaceticus L-arabino-1,5-lactone + reduced N-methylphenazonium methyl sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.35 s-GDH
-
 Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone reconstitution of the apoenzyme to full activity is achieved with a stoichiometric amount of pyrroloquinoline quinone. Mg2+ anchors pyrroloquinoline quinone cofactor to the enzyme protein and activates the bound cofactor Acinetobacter calcoaceticus