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Literature summary extracted from

  • Narumiya, S.; Salmon, J.A.
    Arachidonic acid-15-lipoxygenase from rabbit peritoneal polymorphonuclear leukocytes (1982), Methods Enzymol., 86, 45-48.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.13.11.33 repeated freezing and thawing causes a considerable loss of activity Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.33 5,8,11,14-Eicosatetraynoic acid
-
 Oryctolagus cuniculus
1.13.11.33 BW755C
-
 Oryctolagus cuniculus
1.13.11.33 iodoacetamide 1.0 mM, 30% inhibition Oryctolagus cuniculus
1.13.11.33 PCMB 0.1 mM, 30% inhibition Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.33 0.028
-
 arachidonic acid
-
 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.33 Ca2+ activates reaction with crude extract Oryctolagus cuniculus
1.13.11.33 Mg2+ activates reaction with crude extract Oryctolagus cuniculus
1.13.11.33 Mn2+ activates reaction with crude extract Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.33 61000
-
 gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.33 arachidonate + O2 Oryctolagus cuniculus first step in biotransformation of arachidonic acid to the 15-series of leukotrienes, reaction is involved in inactivation of slow-reacting substrances of anaphylaxis (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.33 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.33
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.33 leukocyte peritoneal polymorphonuclear leukocytes Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.33 93.5
-
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
1.13.11.33 -40°C, 1 month, 60% loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.33 arachidonate + O2
-
 Oryctolagus cuniculus (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate and nonenzymatic production of 15-hydroxy-5,8,11,13-eicosatetraenoic acid, 15-keto-5,8,11,13-eicosatetraenoic acid, 13-hydroxy-14,15-epoxy-5,8,11-eicosatrienoic acid and 11,14,15-trihydroxy-5,8,12-eicosatrienoic acid ?
1.13.11.33 arachidonate + O2 first step in biotransformation of arachidonic acid to the 15-series of leukotrienes, reaction is involved in inactivation of slow-reacting substrances of anaphylaxis Oryctolagus cuniculus (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.33 6.5
-
-
 Oryctolagus cuniculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.33 7
-
 24 h, 30% loss of activity Oryctolagus cuniculus