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Literature summary extracted from

  • Gollub, M.; Schauer, R.; Shaw, L.
    Cytidine monophosphate-N-acetylneuraminate hydroxylase in the starfish Asterias rubens and other echinoderms (1998), Comp. Biochem. Physiol. B, 120, 605-615.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.2 1,10-phenanthroline 2 mM, 88% inhibition Asterias rubens
1.14.18.2 2,2'-dipyridyl 2 mM, 27% inhibition Asterias rubens
1.14.18.2 ferrozine 2 mM, 83% inhibition Asterias rubens
1.14.18.2 KCN 2 mM, 80% inhibition Asterias rubens
1.14.18.2 NaCl above 100 mM Asterias rubens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.2 0.0029
-
 ferrocytochrome b5 pH 6.4, 25°C, amphiphilic form of ferrocytochrome b5 Asterias rubens
1.14.18.2 0.0032
-
 ferrocytochrome b5 pH 6.4, 25°C, soluble form of ferrocytochrome b5 Asterias rubens
1.14.18.2 0.0072
-
 CMP-N-acetylneuraminate pH 6.4, 25°C Asterias rubens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.2 membrane bound to Asterias rubens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.2 FeSO4 activates Asterias rubens
1.14.18.2 NaCl maximal 3.5fold stimulation at 100 mM Asterias rubens
1.14.18.2 NaCl 100 mM activates Ctenodiscus crispatus
1.14.18.2 NaCl 100 mM activates Strongylocentrotus pallidus
1.14.18.2 NaCl 100 mM activates Muelleria sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.2 Asterias rubens
-
-
-
1.14.18.2 Ctenodiscus crispatus
-
-
-
1.14.18.2 Muelleria sp.
-
-
-
1.14.18.2 Strongylocentrotus pallidus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.2 gonad
-
 Asterias rubens
-
1.14.18.2 gonad
-
 Ctenodiscus crispatus
-
1.14.18.2 gonad
-
 Strongylocentrotus pallidus
-
1.14.18.2 gonad
-
 Muelleria sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.2 CMP-N-acetylneuraminate + ferrocytochrome b5 + O2
-
 Asterias rubens CMP-N-glycoloylneuraminate + ferricytrochrome b5 + H2O
-
 ?
1.14.18.2 CMP-N-acetylneuraminate + NADH + O2
-
 Asterias rubens CMP-N-glycoloylneuraminate + NAD+ + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.2 22 27
-
 Asterias rubens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.2 6 6.6
-
 Asterias rubens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.18.2 ferrocytochrome b5
-
 Asterias rubens
1.14.18.2 NADH
-
 Asterias rubens