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Literature summary extracted from

  • Görisch, H.; Lingens, F.
    2-Deoxy-D-arabino-heptulosonate-7-phosphate synthase of Streptomyces aureofaciens Tü 24 (1971), Biochim. Biophys. Acta, 242, 617-629.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.54 dithiothreitol stimulates Kitasatospora aureofaciens

General Stability

EC Number General Stability Organism
2.5.1.54 dithiothreitol stabilizes during incubation at 30°C for 15 min Kitasatospora aureofaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 EDTA reversible by Co2+ Kitasatospora aureofaciens
2.5.1.54 p-hydroxymercuribenzoate
-
 Kitasatospora aureofaciens
2.5.1.54 Phe no inhibition Kitasatospora aureofaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.16
-
 D-erythrose 4-phosphate 30°C, pH 7.0 Kitasatospora aureofaciens
2.5.1.54 0.3
-
 phosphoenolpyruvate 30°C, pH 7.0 Kitasatospora aureofaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Co2+ stimulates Kitasatospora aureofaciens
2.5.1.54 Co2+ required for full activity Kitasatospora aureofaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 97000
-
 gel filtration Kitasatospora aureofaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Kitasatospora aureofaciens first enzyme of the aromatic amino acid biosynthesis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Kitasatospora aureofaciens Tu24 first enzyme of the aromatic amino acid biosynthesis 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Kitasatospora aureofaciens
-
 strain Tü 24
-
2.5.1.54 Kitasatospora aureofaciens Tu24
-
 strain Tü 24
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 170fold Kitasatospora aureofaciens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate ordered ping-pong bi-bi mechanism Kitasatospora aureofaciens
a
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate first substrate is phosphoenolpyruvate Kitasatospora aureofaciens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 1.3
-
 partially purified enzyme Kitasatospora aureofaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Kitasatospora aureofaciens 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O first enzyme of the aromatic amino acid biosynthesis Kitasatospora aureofaciens 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Kitasatospora aureofaciens Tu24 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O first enzyme of the aromatic amino acid biosynthesis Kitasatospora aureofaciens Tu24 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 30
-
 assay at Kitasatospora aureofaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.54 additional information
-
 phosphoenolpyruvate protects against inactivation Kitasatospora aureofaciens
2.5.1.54 additional information
-
 D-erythrose 4-phosphate, erythrose and ribose 5-phosphate increase rate of inactivation Kitasatospora aureofaciens
2.5.1.54 30
-
 pH 6.0-8.0, stable for at least 2 h Kitasatospora aureofaciens
2.5.1.54 50
-
 inactivation above Kitasatospora aureofaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 7
-
-
 Kitasatospora aureofaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.54 6.2 7.9 pH 6.2: about 35% of maximal activity, pH 7.9: about 55% of maximal activity Kitasatospora aureofaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.54 6 8 30°C, stable for at least 2 h Kitasatospora aureofaciens
2.5.1.54 7 7.5 maximal stability Kitasatospora aureofaciens