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Literature summary extracted from

  • Entus, R.; Poling, M.; Herrmann, K.M.
    Redox regulation of Arabidopsis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (2002), Plant Physiol., 129, 1866-1871.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.54 dithiothreitol can partially substitute thioredoxin as activating agent Arabidopsis thaliana
2.5.1.54 reduced thioredoxin required for activity, can be partially substituted by dithiothreitol Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 expression of mature chloroplastic form, without signal sequence, in Escherichia coli Arabidopsis thaliana

General Stability

EC Number General Stability Organism
2.5.1.54 DTT stabilizes the active protein, no influence on the alkylated enzyme Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 iodoacetamide alkylation, abolishes the dependence on reducing agents Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.54 chloroplast chloroplastidic isoenzyme DS-Mn Arabidopsis thaliana 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Mg2+ stimulating, can partly substitute for Mn2+ Arabidopsis thaliana
2.5.1.54 Mn2+ absolutely required, can partly be substituted by Mg2+, but not by Ca2+, Fe2+, and Co2+ Arabidopsis thaliana
2.5.1.54 Mn2+ isozyme DS-Mn Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Arabidopsis thaliana first enzyme in the shikimic pathway leading to biosynthesis of aromatic amino acids 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Arabidopsis thaliana regulatory role of thioredoxin of photosystem I on the chloroplastic enzyme 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 recombinant from Escherichia coli, mature chloroplastic form, purified to homogeneity Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Arabidopsis thaliana 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O first enzyme in the shikimic pathway leading to biosynthesis of aromatic amino acids Arabidopsis thaliana 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O regulatory role of thioredoxin of photosystem I on the chloroplastic enzyme Arabidopsis thaliana 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 37
-
assay at Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.54 25
-
t1/2: 5 h in absence of DTT Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 8.6
-
assay at Arabidopsis thaliana