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Literature summary extracted from

  • Schmid, H.C.; Rassadina, V.; Oster, U.; Schoch, S.; Rüdiger, W.
    Pre-loading of chlorophyll synthase with tetraprenyl diphosphate is an obligatory step in chlorophyll biosynthesis (2002), Biol. Chem., 383, 1769-1778.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.62 D147A mutant enzyme without activity Avena sativa
2.5.1.62 D150A mutant enzyme without activity Avena sativa
2.5.1.62 D154A mutant enzyme without activity Avena sativa
2.5.1.62 N146A mutant enzyme without activity Avena sativa
2.5.1.62 R161A mutant enzyme without activity Avena sativa
2.5.1.62 R161H mutant enzyme with 1% activity compared to wild type enzyme Avena sativa
2.5.1.62 R161K mutant enzyme with 34% activity compared to wild type enzyme Avena sativa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.62 Avena sativa
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recombinant enzyme expressed in Escherichia coli
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Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.62 chlorophyllide a + phytyl diphosphate = chlorophyll a + diphosphate ping-pong mechanism Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.62 chlorophyllide a + tetraprenyl diphosphate ping-pong mechanism. Tetraprenyl diphosphate must bind to the enzyme as the first substrate and esterification occurs when the pre-loaded enzyme meets the second substrate Avena sativa diphosphate + 3-tetraprenylchlorophyllide a
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