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Literature summary extracted from

  • Kim, K.H.; Tchen, T.T.
    Diamine-alpha-ketoglutarate aminotransferase (Escherichia coli) (1971), Methods Enzymol., 17B, 812-815.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.29 cyanide complete inhibition at 0.5 mM Escherichia coli
2.6.1.29 hydroxylamine complete inhibition at 0.5 mM Escherichia coli
2.6.1.29 Semicarbazide complete inhibition at 0.5 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.29 0.88
-
 2-oxoglutarate pH 7.6, room temperature Escherichia coli
2.6.1.29 2.7
-
 pyruvate pH 7.6, room temperature Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.29 putrescine + 2-oxoglutarate Escherichia coli
-
 4-aminobutanal + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.29 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.29 precipitation with ammonium sulphate followed by column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.29 1.63
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.6.1.29 -15ºC, 0.03 M Tris-HCl buffer, pH 7.6, 1 month Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.29 (+)-1,4-diaminobutan-2-ol + 2-oxoglutarate 30% of the activity with putrescine Escherichia coli ?
-
 ?
2.6.1.29 1,6-diaminohexane + 2-oxoglutarate
-
 Escherichia coli 6-aminohexanal + L-glutamate
-
 ?
2.6.1.29 1,7-diaminoheptane + 2-oxoglutarate 30% of the activity with putrescine Escherichia coli 7-aminoheptanal + L-glutamate
-
 ?
2.6.1.29 4-aminobutanoate + 2-oxoglutarate
-
 Escherichia coli 4-oxobutanoate + L-glutamate
-
 ?
2.6.1.29 cadaverine + 2-oxoglutarate equally active as putrescine Escherichia coli 5-aminopentanal + L-glutamate
-
 ?
2.6.1.29 additional information inactive with 1,3-diaminopropane, lysine, ornithine, spermidine Escherichia coli ?
-
 ?
2.6.1.29 additional information inactive with histamine, tyramine, 4-aminobutanol, beta-alanine Escherichia coli ?
-
 ?
2.6.1.29 putrescine + 2-oxoglutarate
-
 Escherichia coli 4-aminobutanal + L-glutamate
-
 ?
2.6.1.29 putrescine + pyruvate
-
 Escherichia coli 4-aminobutanal + L-alanine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.29 9 10
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.29 pyridoxal 5'-phosphate purified enzyme normally active without added pyridoxal phosphate, absolute requirement when irradiated with UV light or treated with 2 mM phenylhydrazine and then with Norite Escherichia coli