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Literature summary extracted from

  • Bajkowski, A.S.; Friedmann, H.C.
    delta-Aminolevulinic acid formation. Purification and properties of alanine:4,5-dioxovalerate, aminotransferase and isolation of 4,5-dioxovalerate from Clostridium tetanomorphum (1982), J. Biol. Chem., 257, 2207-2211.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.43 2-oxoglutarate
-
 Clostridium tetanomorphum
2.6.1.43 amino-oxyacetic acid
-
 Clostridium tetanomorphum
2.6.1.43 diacetyl
-
 Clostridium tetanomorphum
2.6.1.43 Glyoxal
-
 Clostridium tetanomorphum
2.6.1.43 glyoxylic acid
-
 Clostridium tetanomorphum
2.6.1.43 methylglyoxal
-
 Clostridium tetanomorphum
2.6.1.43 oxaloacetic acid
-
 Clostridium tetanomorphum
2.6.1.43 pyruvate
-
 Clostridium tetanomorphum
2.6.1.43 succinate semialdehyde
-
 Clostridium tetanomorphum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.43 0.26
-
 4,5-Dioxopentanoate pH 6.9, 37°C Clostridium tetanomorphum
2.6.1.43 1.96
-
 L-alanine pH 6.9, 37°C Clostridium tetanomorphum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.43 55500
-
 2 * 55500, SDS-PAGE Clostridium tetanomorphum
2.6.1.43 72000
-
 sedimentation equilibrium centrifugation, potassium phosphate buffer containing 4 M NaCl Clostridium tetanomorphum
2.6.1.43 78000
-
 sedimentation equilibrium centrifugation, potassium phosphate buffer Clostridium tetanomorphum
2.6.1.43 82000
-
 gel filtration Clostridium tetanomorphum
2.6.1.43 111000
-
 gel filtration Clostridium tetanomorphum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.43 L-alanine + 4,5-dioxopentanoate Clostridium tetanomorphum
-
 5-aminolevulinate + pyruvate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.43 Clostridium tetanomorphum
-
 ATCC 15920
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.43
-
 Clostridium tetanomorphum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.43 7.68
-
-
 Clostridium tetanomorphum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.43 L-alanine + 4,5-dioxopentanoate
-
 Clostridium tetanomorphum 5-aminolevulinate + pyruvate
-
 ir
2.6.1.43 L-aspartate + 4,5-dioxopentanoate 67% of the activity with L-alanine Clostridium tetanomorphum 5-aminolevulinate + oxaloacetate
-
 ir
2.6.1.43 L-glutamate + 4,5-dioxopentanoate 96% of the activity with L-alanine Clostridium tetanomorphum 5-aminolevulinate + 2-oxoglutarate
-
 ir
2.6.1.43 L-histidine + 4,5-dioxopentanoate 74% of the activity with L-alanine Clostridium tetanomorphum 5-aminolevulinate + 3-(1H-imidazol-4-yl)-2-oxopropanoate
-
 ir
2.6.1.43 L-phenylalanine + 4,5-dioxopentanoate 71% of the activity with L-alanine Clostridium tetanomorphum phenylpyruvate + 5-aminolevulinate
-
 ir

Subunits

EC Number Subunits Comment Organism
2.6.1.43 dimer 2 * 55500, SDS-PAGE Clostridium tetanomorphum

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.43 pyridoxal 5'-phosphate
-
 Clostridium tetanomorphum