Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Isaac, J.E.; Rhodes, M.J.C.
    Purification and properties of phosphofructokinase from fruits of Lycopersicon esculentum (1982), Phytochemistry, 21, 1553-1556.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.11 dithioerythritol stabilizes Solanum lycopersicum
2.7.1.11 MgATP2- or ATP and Mg2+ stabilize during purification Solanum lycopersicum
2.7.1.11 sensitive to freezing, complete inactivation after 24 h at -20°C at pH 7.5 Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 Cl-
-
 Solanum lycopersicum
2.7.1.11 NO2-
-
 Solanum lycopersicum
2.7.1.11 NO3-
-
 Solanum lycopersicum
2.7.1.11 SO42-
-
 Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 Mg2+ required for activity Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 35000
-
 gel filtration at pH 8 Solanum lycopersicum
2.7.1.11 180000
-
 major form, gel filtration at pH 7.5 or 8.0 Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate Solanum lycopersicum allosteric enzyme, involved in regulation of glycolytic flux ADP + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Solanum lycopersicum
-
 tomato, var. Eurocross BB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.11 Blue Sepharose, ATP-Sepharose Solanum lycopersicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.11 0.61
-
-
 Solanum lycopersicum

Storage Stability

EC Number Storage Stability Organism
2.7.1.11 -20°C, inactivation within 24 h Solanum lycopersicum
2.7.1.11 0-4°C, more stable in Tris than in phosphate buffer Solanum lycopersicum
2.7.1.11 enzyme extracts prepared at pH 8 are less stable to storage at 0-4°C than extracts prepared at pH 7.5 Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Solanum lycopersicum ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate allosteric enzyme, involved in regulation of glycolytic flux Solanum lycopersicum ADP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 25
-
 assay at Solanum lycopersicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.11 60
-
 3 min, inactivation, enzyme prepared by ATP-Sepharose chromatography Solanum lycopersicum
2.7.1.11 100
-
 5 min, 70% loss of activity, enzyme prepared by DEAE-cellulose chromatography in the absence of MgATP Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 8 8.3
-
 Solanum lycopersicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.11 7.5 8.3 approx. half-maximal activity at pH 7.5, maximal activity at pH 8.3, approx. 15% of maximal activity at pH 7 Solanum lycopersicum