EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.20 | adenosine | kinetics | Oryctolagus cuniculus | |
2.7.1.20 | ATP | - |
Oryctolagus cuniculus | |
2.7.1.20 | EDTA | at high concentrations | Oryctolagus cuniculus | |
2.7.1.20 | Mg2+ | - |
Oryctolagus cuniculus | |
2.7.1.20 | additional information | no inhibition by PMSF, EGTA | Oryctolagus cuniculus | |
2.7.1.20 | p-hydroxymercuribenzoate | DTT reverses | Oryctolagus cuniculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.20 | 0.00015 | 0.0005 | adenosine | pH 6.8, 30ºC | Oryctolagus cuniculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.20 | cytosol | - |
Oryctolagus cuniculus | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.20 | Ba2+ | no activation | Oryctolagus cuniculus | |
2.7.1.20 | Ca2+ | activation | Oryctolagus cuniculus | |
2.7.1.20 | Mg2+ | requirement | Oryctolagus cuniculus | |
2.7.1.20 | Mn2+ | can partially substitute for Mg2+ | Oryctolagus cuniculus | |
2.7.1.20 | Mn2+ | activation | Oryctolagus cuniculus | |
2.7.1.20 | additional information | emission spectral analysis show that enzyme contains tightly bound divalent metal ions | Oryctolagus cuniculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.20 | 34500 | - |
gel filtration | Oryctolagus cuniculus |
2.7.1.20 | 51000 | - |
1 * 51000, SDS-PAGE | Oryctolagus cuniculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.20 | ATP + adenosine | Oryctolagus cuniculus | involved in phosphorylation of many pharmacological active purine nucleosides | ADP + AMP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.20 | Oryctolagus cuniculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.20 | gel filtration and affinity chromatography | Oryctolagus cuniculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.20 | liver | - |
Oryctolagus cuniculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.20 | 1.75 | 2.23 | - |
Oryctolagus cuniculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.20 | ATP + adenosine | specificity | Oryctolagus cuniculus | ADP + AMP | - |
? | |
2.7.1.20 | ATP + adenosine | best substrates | Oryctolagus cuniculus | ADP + AMP | - |
? | |
2.7.1.20 | ATP + adenosine | ATP-analogues are also substrates | Oryctolagus cuniculus | ADP + AMP | - |
? | |
2.7.1.20 | ATP + adenosine | involved in phosphorylation of many pharmacological active purine nucleosides | Oryctolagus cuniculus | ADP + AMP | - |
? | |
2.7.1.20 | CTP + adenosine | CTP is a poor substrate | Oryctolagus cuniculus | CDP + AMP | - |
? | |
2.7.1.20 | GTP + adenosine | - |
Oryctolagus cuniculus | GDP + AMP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.20 | monomer | 1 * 51000, SDS-PAGE | Oryctolagus cuniculus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.20 | 30 | - |
assay at | Oryctolagus cuniculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.20 | additional information | - |
the pH-optimum is a function of the adenosine concentration | Oryctolagus cuniculus |
2.7.1.20 | additional information | - |
the pH-optimum is a function of the ATP/Mg2+ ratio | Oryctolagus cuniculus |
2.7.1.20 | 4.8 | - |
below, ATP/Mg2+ ratio: 0.1 | Oryctolagus cuniculus |
2.7.1.20 | 5.2 | - |
ATP/Mg2+ ratio: 1 | Oryctolagus cuniculus |
2.7.1.20 | 5.5 | - |
ATP/Mg2+ ratio: 10 | Oryctolagus cuniculus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.20 | 4.6 | 5.8 | about half-maximal activity at pH 4.6 and pH 5.8, ATP/Mg2+ ratio: 1 | Oryctolagus cuniculus |
2.7.1.20 | 5.2 | 6.4 | about half-maximal activity at pH 5.2 and pH 6.4, ATP/Mg2+ ratio: 10 | Oryctolagus cuniculus |